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Granzyme A Peptide 1

Antibody: CPTC-GZMA-1
Background

Catalog Number:

CPTC-GZMA-1

RRID:

AB_2890081

Target Antigen:

Granzyme A Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-GZMA-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6211) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-GZMA-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-GZMA-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6211)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array

CTPC-GZMA-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-GZMA-1 as primary antibody against human granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) recombinant protein (lane 2). Expected molecular weight - 28.8 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-GZMA-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-GZMA-1 as primary antibody against human granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) recombinant protein (lane 2). Expected molecular weight - 28.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-GZMA-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 29.0 kDa and 27.1 kDa. Molecular weight standards are also included (lane 1). NCI-H226 is positive. All other cell lines are negative. Click image to enlarge

CPTC-GZMA-1 Western Blot Cell Lysate

Result: Positive

Western blot using CPTC-GZMA-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 29.0 kDa and 27.1 kDa. Molecular weight standards are also included (lane 1).
NCI-H226 is positive. All other cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-GZMA-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 29.0 kDa and 27.1 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-GZMA-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-GZMA-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 29.0 kDa and 27.1 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Granzyme A Peptide 1
Background

NCI Identification Number:

00366

Antigen Name:

Granzyme A Peptide 1

CPTC Name:

CPTC-GZMA Peptide 1

Aliases:

Granzyme A; CTL Tryptase; Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated Serine Esterase 3); Cytotoxic T-Lymphocyte-Associated Serine Esterase-3; Cytotoxic T-Lymphocyte Proteinase 1; Hanukah Factor Serine Protease; Fragmentin-1; Granzyme 1; H Factor; CTLA3; HFSP; HF; Granzyme A (Cytotoxic T-Lymphocyte-Associated Serine Esterase-3; Hanukah Factor Serine Protease); Hanukkah Factor; EC 3.4.21; EC 3.4.21.78; Granzyme-1; GZMA

Function:

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.GZMA (Granzyme A) is a Protein Coding gene. Diseases associated with GZMA include Chediak-Higashi Syndrome and Smallpox. Among its related pathways are Common Cytokine Receptor Gamma-Chain Family Signaling Pathways and Granzyme-A Pathway. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and serine-type peptidase activity. An important paralog of this gene is GZMK.Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.

Chromosomal Localization:

5q11.2

Accession Number:

NP_006135.2

UniProt Accession Number:

P12544

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

EFPYPCYDPATR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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