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Glutathione S-Transferase Mu2

Antibody: CPTC-GST Mu2-1
Background

Catalog Number:

CPTC-GST Mu2-1

RRID:

AB_10659455

Target Antigen:

Glutathione S-Transferase Mu2

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2021

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image Kinetic titration data for GSTMu2-1 Ab (623A2.1) using Biacore SPR method Click image to enlarge

CPTC-GSTMu2-1 SPR

Result: High Binding

Kinetic titration data for GSTMu2-1 Ab (623A2.1) using Biacore SPR method


Characterization SOP Files

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens. Click image to enlarge

Cross Reactivity Data

Result: Positive

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (111.7 KB)

CPTC-GSTMu2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu2-1 Indirect ELISA

Result: High Binding

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-GSTMu2-1 as primary Ab against Ag 10112 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu2-1 Western Blot

Result: Negative

Western Blot using CPTC-GSTMu2-1 as primary Ab against Ag 10112 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-GST Mu2-2
Background

Catalog Number:

CPTC-GST Mu2-2

RRID:

AB_10660287

Target Antigen:

Glutathione S-Transferase Mu2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/03/2026

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens. Click image to enlarge

Cross Reactivity Data

Result: Positive

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.


Characterization SOP Files

  CyTOF
Click to enlarge image Imaging mass cytometry on lung cancer tissue core using CPTC-GSTMu2-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, liver, colon, endometrium, appendix, bone marrow, breast, kidney, lung, and placenta) and cancer tissues (breast, colon, prostate, lung, and ovarian). Click image to enlarge

CPTC-GSTMu2-2 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on lung cancer tissue core using CPTC-GSTMu2-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, liver, colon, endometrium, appendix, bone marrow, breast, kidney, lung, and placenta) and cancer tissues (breast, colon, prostate, lung, and ovarian).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (166.0 KB)

CPTC-GSTMu2-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles. Human assay: A-431 fixed with PFA, dilution: 1:20 Human assay: U-2 OS fixed with PFA, dilution: 1:20 Human assay: U-251 MG fixed with PFA, dilution: 1:20 Click image to enlarge

CPTC-GSTMu2-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles. Human assay: A-431 fixed with PFA, dilution: 1:20
Human assay: U-2 OS fixed with PFA, dilution: 1:20
Human assay: U-251 MG fixed with PFA, dilution: 1:20


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu2-2 Indirect ELISA

Result: High Binding

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-GSTMu2-2 as primary Ab against Ag 10112 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu2-2 Western Blot

Result: Positive

Western Blot using CPTC-GSTMu2-2 as primary Ab against Ag 10112 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-GST Mu2-3
Background

Catalog Number:

CPTC-GST Mu2-3

RRID:

AB_2116064

Target Antigen:

Glutathione S-Transferase Mu2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2021

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens. Click image to enlarge

Cross Reactivity Data

Result: Positive

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (109.0 KB)

CPTC-GSTMu2-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu2-3 Indirect ELISA

Result: High Binding

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-GSTMu2-3 as primary Ab against Ag 10112 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu2-3 Western Blot

Result: Positive

Western Blot using CPTC-GSTMu2-3 as primary Ab against Ag 10112 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Glutathione S-Transferase Mu2
Background

NCI Identification Number:

10112

Antigen Name:

Glutathione S-Transferase Mu2

CPTC Name:

CPTC-GST Mu2

Aliases:

GSTM2; EC 2.5.1.18; GST4; GSTM; GSTM2-2; GTHMUS; MGC117303; OTTHUMP00000013351

Function:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles

Chromosomal Localization:

1p13.3

Expression System:

E. Coli

Accession Number:

NM_000848

UniProt Accession Number:

P28161

DNA Source:

HIP:HsCD00000624

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

39943

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMPMTLGYWNIRGLAHSIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWL
NEKFKLGLDFPNLPYLIDGTHKITQSNAILRYIARKHNLCGESEKEQIRE
DILENQFMDSRMQLAKLCYDPDFEKLKPEYLQALPEMLKLYSQFLGKQPW
FLGDKITFVDFIAYDVLERNQVFEPSCLDAFPNLKDFISRFEGLEKISAY
MKSSRFLPRPVFTKMAVWGNK

Native Sequence:

MPMTLGYWNIRGLAHSIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEK
FKLGLDFPNLPYLIDGTHKITQSNAILRYIARKHNLCGESEKEQIREDIL
ENQFMDSRMQLAKLCYDPDFEKLKPEYLQALPEMLKLYSQFLGKQPWFLG
DKITFVDFIAYDVLERNQVFEPSCLDAFPNLKDFISRFEGLEKISAYMKS
SRFLPRPVFTKMAVWGNK

Calculated Isoelectric Point:

5.99

Molecular Weight:

26017

Last Updated:

08/22/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of Ag 10112 (with molecular weight standards in lane 1)
Click image to enlarge

CPTC-GSTMu2

PAGE of Ag 10112 (with molecular weight standards in lane 1)

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SOPs

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