Background
Catalog Number:
CPTC-GST Mu2-1
RRID:
AB_10659455
Target Antigen:
Glutathione S-Transferase Mu2
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
08/24/2021
Antigen Recognition(s):
Recombinant Full-length
SOP:
Purchase
- DSHB CPTC-GST Mu2-1
Characterization Data [Compare Characterization Data]
Affinity Measurement by SPR
CPTC-GSTMu2-1 SPR
Result: Positive
Kinetic titration data for GSTMu2-1 Ab (623A2.1) using Biacore SPR method
Characterization SOP Files
Cross Reactivity Data
Cross Reactivity Data
Result: Positive
This table shows the cross reactivity by indirect ELISA with the GST group of antigens.
Characterization SOP Files
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (111.7 KB)
CPTC-GSTMu2-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-GSTMu2-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-GSTMu2-1 Western Blot
Result: Negative
Western Blot using CPTC-GSTMu2-1 as primary Ab against Ag 10112 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-GST Mu2-2
RRID:
AB_10660287
Target Antigen:
Glutathione S-Transferase Mu2
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-GST Mu2-2
External Links
- Human Protein Atlas CPTC-GST Mu2-1
Characterization Data [Compare Characterization Data]
Cross Reactivity Data
Cross Reactivity Data
Result: Positive
This table shows the cross reactivity by indirect ELISA with the GST group of antigens.
Characterization SOP Files
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (166.0 KB)
CPTC-GSTMu2-2 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immunofluorescence - HPA
CPTC-GSTMu2-2 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles. Human assay: A-431 fixed with PFA, dilution: 1:20
Human assay: U-2 OS fixed with PFA, dilution: 1:20
Human assay: U-251 MG fixed with PFA, dilution: 1:20
Characterization SOP Files
Indirect ELISA
CPTC-GSTMu2-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-GSTMu2-2 Western Blot
Result: Positive
Western Blot using CPTC-GSTMu2-2 as primary Ab against Ag 10112 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-GST Mu2-3
RRID:
AB_2116064
Target Antigen:
Glutathione S-Transferase Mu2
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/24/2021
Antigen Recognition(s):
Recombinant Full-length
SOP:
Purchase
- DSHB CPTC-GST Mu2-3
Characterization Data [Compare Characterization Data]
Cross Reactivity Data
Cross Reactivity Data
Result: Positive
This table shows the cross reactivity by indirect ELISA with the GST group of antigens.
Characterization SOP Files
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (109.0 KB)
CPTC-GSTMu2-3 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-GSTMu2-3 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-GSTMu2-3 Western Blot
Result: Positive
Western Blot using CPTC-GSTMu2-3 as primary Ab against Ag 10112 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
10112
Antigen Name:
Glutathione S-Transferase Mu2
CPTC Name:
CPTC-GST Mu2
Aliases:
GSTM2; EC 2.5.1.18; GST4; GSTM; GSTM2-2; GTHMUS; MGC117303; OTTHUMP00000013351
Function:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles
Chromosomal Localization:
1p13.3
Expression System:
E. Coli
Accession Number:
NM_000848
UniProt Accession Number:
P28161
DNA Source:
HIP:HsCD00000624
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
39943
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMPMTLGYWNIRGLAHSIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWL
NEKFKLGLDFPNLPYLIDGTHKITQSNAILRYIARKHNLCGESEKEQIRE
DILENQFMDSRMQLAKLCYDPDFEKLKPEYLQALPEMLKLYSQFLGKQPW
FLGDKITFVDFIAYDVLERNQVFEPSCLDAFPNLKDFISRFEGLEKISAY
MKSSRFLPRPVFTKMAVWGNK
Native Sequence:
MPMTLGYWNIRGLAHSIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEK
FKLGLDFPNLPYLIDGTHKITQSNAILRYIARKHNLCGESEKEQIREDIL
ENQFMDSRMQLAKLCYDPDFEKLKPEYLQALPEMLKLYSQFLGKQPWFLG
DKITFVDFIAYDVLERNQVFEPSCLDAFPNLKDFISRFEGLEKISAYMKS
SRFLPRPVFTKMAVWGNK
Calculated Isoelectric Point:
5.99
Molecular Weight:
26017
Last Updated:
08/22/2020
Links
- EnsEMBL - EnsEMBL GSTM2
- Entrez - Entrez GSTMu2
- PlasmID - CPTC-GSTMu2.v
- Uniprot - Uniprot P28161
Characterization Data
Gel
Click image to enlarge
CPTC-GSTMu2
PAGE of Ag 10112 (with molecular weight standards in lane 1)
Characterization SOP Files
SOPs
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