Background
Catalog Number:
CPTC-GSC-3
Target Antigen:
Goosecoid Homeobox
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
12/31/2020
Antigen Recognition(s):
Recombinant Full-length
Thematic Panel(s):
Epithelial Mesenchymal Transition (EMT)
Publications
Characterization Data [Compare Characterization Data]
IP
Download Immuno-precipitation data obtained incubating CPTC-GSC-3 and the recombinant GSC (aa1-257, expected, MW ~30KDa) (77.2 KB)
CPTC-GSC-3 IPMS
Result: Negative
Immuno-precipitation data obtained incubating CPTC-GSC-3 and the recombinant GSC (aa1-257, expected, MW ~30KDa)
Indirect ELISA
CPTC-GSC-3 Indirect ELISA
Result: Positive
Indirect ELISA using CPTC-GSC-3 as primary antibody against recombinant GSC protein (amino acids 1-257).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-GSC-3 recombinant protein WB
Result: Negative
WB using CPTC-GSC-3 as primary antibody against recombinant GSC protein (amino acids 1-257) with expected MW of 30.9 KDa.
Characterization SOP Files
Background
Catalog Number:
CPTC-GSC-4
RRID:
AB_2827866
Target Antigen:
Goosecoid Homeobox
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/06/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length
Thematic Panel(s):
Epithelial Mesenchymal Transition (EMT)
Purchase
- DSHB CPTC-GSC-4
Publications
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (341.3 KB)
CPTC-GSC-4 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IP
Download Immuno-precipitation data obtained incubating CPTC-GSC-4 and the recombinant GSC (aa1-257, expected, MW ~30KDa) (81.5 KB)
CPTC-GSC-4
Result: Positive
Immuno-precipitation data obtained incubating CPTC-GSC-4 and the recombinant GSC (aa1-257, expected, MW ~30KDa)
Indirect ELISA
CPTC-GSC-4 Indirect ELISA
Result: Positive
Indirect ELISA using CPTC-GSC-4 as primary antibody against recombinant GSC protein (amino acids 1-257).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-GSC-4 recombinant protein WB
Result: Negative
WB using CPTC-GSC-4 as primary antibody against recombinant GSC protein (amino acids 1-257) with expected MW of 30.9 KDa.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-GSC-4 Endogenous Western Blot
Result: Negative
Western Blot using CPTC-GSC-4 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 28.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-GSC-4 Endogenous Western Blot
Result: Negative
Automated Western Blot using CPTC-GSC-4 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 28.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00067
Antigen Name:
Goosecoid Homeobox
CPTC Name:
CPTC-GSC
Aliases:
Homeobox Protein Goosecoid
Function:
This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.
GSC (goosecoid homeobox) is a protein-coding gene. Diseases associated with GSC include miller fisher syndrome, and auditory neuropathy. GO annotations related to this gene include RNA polymerase II transcription factor binding and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is PITX1.
Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring.
Chromosomal Localization:
14q32.13
Accession Number:
NP_776248.1
UniProt Accession Number:
P56915
DNA Source:
The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
Buffers:
Expressed Sequence:
MHHHHHHSSGVDLGTENLYFQSNAMPASMFSIDNILAARPRCKDSVLPVA
HSAAAPVVFPALHGDSLYGASGGASSDYGAFYPRPVAPGGAGLPAAVSGS
RLGYNNYFYGQLHVQAAPVGPACCGAVPPLGAQQCSCVPTPPGYEGPGSV
LVSPVPHQMLPYMNVGTLSRTELQLLNQLHCRRKRRHRTIFTDEQLEALE
NLFQETKYPDVGTREQLARKVHLREEKVEVWFKNRRAKWRRQKRSSSEES
ENAEKWNKTSSSKASPEKREEEGKSDLDSDS
Native Sequence:
MHHHHHHSSGVDLGTENLYFQSNAMPASMFSIDNILAARPRCKDSVLPVA
HSAAAPVVFPALHGDSLYGASGGASSDYGAFYPRPVAPGGAGLPAAVSGS
RLGYNNYFYGQLHVQAAPVGPACCGAVPPLGAQQCSCVPTPPGYEGPGSV
LVSPVPHQMLPYMNVGTLSRTELQLLNQLHCRRKRRHRTIFTDEQLEALE
NLFQETKYPDVGTREQLARKVHLREEKVEVWFKNRRAKWRRQKRSSSEES
ENAEKWNKTSSSKASPEKREEEGKSDLDSDS
Calculated Isoelectric Point:
0
Molecular Weight:
100
Last Updated:
09/16/2019
Links
- Entrez - Entrez GSC
- EnsEMBL - EnsEMBL GSC
- Uniprot - Uniprot P56915
Characterization Data
SOPs
No SOPs available.
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