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Goosecoid Homeobox (Epitope recognition PASMFSIDNILAARPRC, aa 2-18)

Antibody: CPTC-GSC-1
Background

Catalog Number:

CPTC-GSC-1

Target Antigen:

Goosecoid Homeobox (Epitope recognition PASMFSIDNILAARPRC, aa 2-18)

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (346.9 KB)

CPTC-GSC (Epitope aa 2-18)-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Download Immuno-precipitation data obtained incubating CPTC-GSC-1 and the recombinant GSC (aa1-257, expected, MW ~30KDa) (81.2 KB)

CPTC-GSC-1 IPMS

Result: Positive

Immuno-precipitation data obtained incubating CPTC-GSC-1 and the recombinant GSC (aa1-257, expected, MW ~30KDa)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-GSC-1 as primary antibody against recombinant GSC protein (amino acids 1-257). Click image to enlarge

CPTC-GSC-1 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-GSC-1 as primary antibody against recombinant GSC protein (amino acids 1-257).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-GSC-1 recombinant protein WB

Result: Negative

WB using CPTC-GSC-1 as primary antibody against recombinat GSC protein (amino acids 1-257) with expected MW of 30.9 KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-GSC-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 28.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-GSC-1 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-GSC-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 28.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-GSC-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 28.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-GSC-1 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-GSC-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 28.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antibody: CPTC-GSC-2
Background

Catalog Number:

CPTC-GSC-2

RRID:

AB_2827837

Target Antigen:

Goosecoid Homeobox (Epitope recognition PASMFSIDNILAARPRC, aa 2-18)

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (407.6 KB)

CPTC-GSC-2 evaluation by the Human Protein Atlas

Result: Negative

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IP
Download Immuno-precipitation data obtained incubating CPTC-GSC-2 and the recombinant GSC (aa1-257, expected, MW ~30KDa) (79.3 KB)

CPTC-GSC-2 IPMS

Result: Negative

Immuno-precipitation data obtained incubating CPTC-GSC-2 and the recombinant GSC (aa1-257, expected, MW ~30KDa)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-GSC-2 as primary antibody against recombinant GSC protein (amino acids 1-257). Click image to enlarge

CPTC-GSC-2 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-GSC-2 as primary antibody against recombinant GSC protein (amino acids 1-257).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC- GSC-2 recombinant protein WB

Result: Negative

WB using CPTC-GSC-2 as primary antibody against recombinat GSC protein (amino acids 1-257) with expected MW of 30.9 KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-GSC-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 28.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-GSC-2 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-GSC-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 28.1 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-GSC-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 28.1 KDa. All cell lysates negative/incoclusive. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-GSC-2 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-GSC-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 28.1 KDa. All cell lysates negative/incoclusive. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Goosecoid Homeobox (Epitope recognition PASMFSIDNILAARPRC, aa 2-18)
Background

NCI Identification Number:

00066

Antigen Name:

Goosecoid Homeobox (Epitope recognition PASMFSIDNILAARPRC, aa 2-18)

CPTC Name:

CPTC-GSC (Epitope aa 2-18)

Aliases:

Homeobox Protein Goosecoid

Function:

This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.

GSC (goosecoid homeobox) is a protein-coding gene. Diseases associated with GSC include miller fisher syndrome, and auditory neuropathy. GO annotations related to this gene include RNA polymerase II transcription factor binding and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is PITX1.

Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring.

Chromosomal Localization:

14q32.13

Accession Number:

NP_776248.1

UniProt Accession Number:

P56915

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

Buffers:

Expressed Sequence:

MHHHHHHSSGVDLGTENLYFQSNAMPASMFSIDNILAARPRCKDSVLPVA
HSAAAPVVFPALHGDSLYGASGGASSDYGAFYPRPVAPGGAGLPAAVSGS
RLGYNNYFYGQLHVQAAPVGPACCGAVPPLGAQQCSCVPTPPGYEGPGSV
LVSPVPHQMLPYMNVGTLSRTELQLLNQLHCRRKRRHRTIFTDEQLEALE
NLFQETKYPDVGTREQLARKVHLREEKVEVWFKNRRAKWRRQKRSSSEES
ENAEKWNKTSSSKASPEKREEEGKSDLDSDS

Native Sequence:

MPASMFSIDNILAARPRCKDSVLPVAHSAAAPVVFPALHGDSLYGASGGA
SSDYGAFYPRPVAPGGAGLPAAVSGSRLGYNNYFYGQLHVQAAPVGPACC
GAVPPLGAQQCSCVPTPPGYEGPGSVLVSPVPHQMLPYMNVGTLSRTELQ
LLNQLHCRRKRRHRTIFTDEQLEALENLFQETKYPDVGTREQLARKVHLR
EEKVEVWFKNRRAKWRRQKRSSSEESENAEKWNKTSSSKASPEKREEEGK
SDLDSDS

Calculated Isoelectric Point:

0

Molecular Weight:

31000

Last Updated:

09/16/2019

Links

Characterization Data

SOPs

No SOPs available.

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