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Glyceraldehyde-3-Phosphate Dehydrogenase Peptide 1

Antibody: CPTC-GAPDH-1
Background

Catalog Number:

CPTC-GAPDH-1

Target Antigen:

Glyceraldehyde-3-Phosphate Dehydrogenase Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

External Links
Publications
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-GAPDH-1 and full-length GAPDH recombinant protein were measured using surface plasmon resonance. CPTC-GADPH-1 antibody was captured on protein A biosensor chip. GADPH recombinant protein at 1024 nM, 256 nM, 64 nM, 16 nM, and 4 nM, was used as analyte. All binding data were double referenced and analyzed globally using a 1:1 fitting model. Click image to enlarge

CPTC-GAPDH-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Positive

Affinity and binding kinetics of CPTC-GAPDH-1 and full-length GAPDH recombinant protein were measured using surface plasmon resonance. CPTC-GADPH-1 antibody was captured on protein A biosensor chip. GADPH recombinant protein at 1024 nM, 256 nM, 64 nM, 16 nM, and 4 nM, was used as analyte. All binding data were double referenced and analyzed globally using a 1:1 fitting model.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (402.1 KB)

CPTC-GAPDH-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and nuclear staining using Antibody CPTC-GAPDH-1. Titer: 1:20000 Click image to enlarge

CTPC-GAPDH-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and nuclear staining using Antibody CPTC-GAPDH-1. Titer: 1:20000


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and nuclear staining using Antibody CPTC-GAPDH-1. Titer: 1:20000 Click image to enlarge

CTPC-GAPDH-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and nuclear staining using Antibody CPTC-GAPDH-1. Titer: 1:20000


Characterization SOP Files

  IP
Click to enlarge image Immuno-precipitation performed using CPTC-GAPDH-3 as capture antibody against the over-expressed lysate of GAPDH (Myc-tagged). Eluates were tested in automated western blot using the an anti-Myc tag as detection antibody. The target protein was pulled down. Click image to enlarge

CPTC-GAPDH-1 Immunoprecipitation

Result: Positive

Immuno-precipitation performed using CPTC-GAPDH-3 as capture antibody against the over-expressed lysate of GAPDH (Myc-tagged). Eluates were tested in automated western blot using the an anti-Myc tag as detection antibody. The target protein was pulled down.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image CPTC-GAPDH-1 iMRM Click image to enlarge

CPTC-GAPDH-1 iMRM

Result: Positive

CPTC-GAPDH-1 iMRM


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of human cell line HeLa with CPTC-GAPDH-1 Ab shows localization to the mitochondrion, nucleus, cytoskeleton, other. Click image to enlarge

CPTC-GAPDH-1

Result: Positive

Immunofluorescence staining of human cell line HeLa with CPTC-GAPDH-1 Ab shows localization to the mitochondrion, nucleus, cytoskeleton, other.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-GAPDH-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CTPC-GAPDH-1 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-GAPDH-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-GAPDH-1 as primary antibody against GAPDH recombinant protein (lane 2). Also included are molecular weight standards (lane 1) Click image to enlarge

CPTC-GAPDH-1 Western Blot (recombinant protein)

Result: Positive

Western Blot using CPTC-GAPDH-1 as primary antibody against GAPDH recombinant protein (lane 2). Also included are molecular weight standards (lane 1)


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-GAPDH-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1) Click image to enlarge

CPTC-GAPDH-1 Western Blot (cell lysates)

Result: Positive

Western Blot using CPTC-GAPDH-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1)


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western Blot using CPTC-GAPDH-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1) Click image to enlarge

CPTC-GAPDH-1 Automated Western Blot (cell lyates)

Result: Positive

Automated western Blot using CPTC-GAPDH-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1)


Characterization SOP Files

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Antigen: Glyceraldehyde-3-Phosphate Dehydrogenase Peptide 1
Background

NCI Identification Number:

00307

Antigen Name:

Glyceraldehyde-3-Phosphate Dehydrogenase Peptide 1

CPTC Name:

CPTC-GAPDH Peptide 1

Aliases:

Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein; OCAS, P38 Component; HEL-S-162eP; EC 2.6.99.-; EC 1.2.1; G3PD

Function:

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is a Protein Coding gene. Diseases associated with GAPDH include Fmr1-Related Disorders and Schistosomiasis. Among its related pathways are Glucose metabolism and Neuroscience. Gene Ontology (GO) annotations related to this gene include identical protein binding and NAD binding. An important paralog of this gene is GAPDHS.Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Chromosomal Localization:

12p13.31

Accession Number:

NP_002037.2

UniProt Accession Number:

P04406

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CGALQNIIPASTGAAK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1759

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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