Forkhead Box O3 Peptide 1


Background

Catalog Number:

CPTC-FOXO3-1

Target Antigen:

Forkhead Box O3 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/05/2021

Antigen Recognition(s):

Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

Publications
Characterization Data [Compare Characterization Data]
  IPMS
Download Immuno-precipitation data obtained incubating CPTC-FOXO3-1 and the recombinant domain of FOXO3 (aa10-170, expected, MW ~16KDa) (139.6 KB)

CPTC-FOXO3-1 IPMS

Result: Negative

Immuno-precipitation data obtained incubating CPTC-FOXO3-1 and the recombinant domain of FOXO3 (aa10-170, expected, MW ~16KDa)


  Western Blot - Cell Lysate
Click to enlarge image Western Blot using CPTC-FOXO3-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 71.2 KDa. All cell lysates negative.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-FOXO3-1 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-FOXO3-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 71.2 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-FOXO3-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 71.3 KDa. All cell lysates negative.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-FOXO3-1 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-FOXO3-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 71.3 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-FOXO3-1 as primary antibody against FOXO3 protein domain comprising amino acids 10-170 (lane 2) with expected MW of 16.5 KDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-FOXO3-1 recombinant protein WB

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-FOXO3-1 as primary antibody against FOXO3 protein domain comprising amino acids 10-170 (lane 2) with expected MW of 16.5 KDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-FOXO3-1 as primary antibody against FOXO3 recombinant protein  (lane 2) with expected MW of 71.3 KDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-FOXO3-1 recombinant protein WB

Result: Positive

Western Blot using CPTC-FOXO3-1 as primary antibody against FOXO3 recombinant protein (lane 2) with expected MW of 71.3 KDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

Background

NCI Identification Number:

00268

Antigen Name:

Forkhead Box O3 Peptide 1

CPTC Name:

CPTC-FOXO3 Peptide 1

Keywords:

Forkhead Box O3; Forkhead In Rhabdomyosarcoma-Like 1; FKHRL1; FOXO3A; Forkhead, Drosophila, Homolog Of, In Rhabdomyosarcoma-Like 1; Forkhead Homolog (Rhabdomyosarcoma) Like 1; Forkhead Box Protein O3; Forkhead Box O3A; AF6q21 Protein; FKHRL1P2; AF6q21; FOXO2

Function:

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.FOXO3 (Forkhead Box O3) is a Protein Coding gene. Diseases associated with FOXO3 include Chromosome 6Q Deletion and Rhabdomyosarcoma. Among its related pathways are Common Cytokine Receptor Gamma-Chain Family Signaling Pathways and RET signaling. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and protein kinase binding. An important paralog of this gene is FOXO1.ranscriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed:10102273, PubMed:16751106, PubMed:21329882). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity).

Chromosomal Localization:

6q21

NCBI Accession Number:

NP_001446.1

UniProt Accession Number:

O43524

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SPAKPSGETAADSMIPEEEc

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2090

Last Updated:

10/23/2020

Links

Characterization Data

SOPs

No SOPs available.

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