Background
Catalog Number:
CPTC-FOXO1-1
Target Antigen:
Forkhead Box O1 Peptide 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
02/06/2024
Antigen Recognition(s):
Peptide, Endogenous
Purchase
- DSHB CPTC-FOXO1-1
Characterization Data [Compare Characterization Data]
IHC Tissue
CPTC-FOXO1-1 IHC Tissue
Result: Presumed Positive
Tissue Micro-Array (TMA) core of colon showing cytoplasmic staining using Antibody CPTC-FOXO1-1. Titer: 1:1000
The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity.
Characterization SOP Files
NCI 60 Protein Array
CPTC-FOXO1-1 NCI 60 Protein Array
Result: Positive
Protein Array in which CPTC-FOXO1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-FOXO1-1 Western Blot Recombinant Protein
Result: Presumed Positive (with additional bands)
Western blot using CPTC-FOXO1-1 as primary antibody against human forkhead box O1 (FOXO1) recombinant protein (lane 2). Expected molecular weight - 69.5 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-FOXO1-1 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-FOXO1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 69.7 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-FOXO1-1 Western Blot Cell Lysate
Result: Positive
Western blot using CPTC-FOXO1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 69.7 kDa. Molecular weight standards are also included (lane 1). All cell lines are positive except for PBMC.
Characterization SOP Files
Background
Catalog Number:
CPTC-FOXO1-2
Target Antigen:
Forkhead Box O1 Peptide 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/10/2022
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-FOXO1-3
Target Antigen:
Forkhead Box O1 Peptide 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/10/2022
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
Background
NCI Identification Number:
00473
Antigen Name:
Forkhead Box O1 Peptide 1
CPTC Name:
CPTC-FOXO1 Peptide 1
Aliases:
Forkhead Box O1; Forkhead Box Protein O1A; Forkhead Box Protein O1; FOXO1A; FKH1; FKHR; Forkhead, Drosophila, Homolog Of, In Rhabdomyosarcoma; Forkhead Homolog In Rhabdomyosarcoma; Forkhead In Rhabdomyosarcoma; FOXO1
Function:
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. FOXO1 (Forkhead Box O1) is a Protein Coding gene. Diseases associated with FOXO1 include Rhabdomyosarcoma 2 and Glioma. Among its related pathways are Common Cytokine Receptor Gamma-Chain Family Signaling Pathways and Transcriptional misregulation in cancer. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and chromatin binding. An important paralog of this gene is FOXO3.Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress (PubMed:10358076, PubMed:12228231, PubMed:15220471, PubMed:15890677, PubMed:18356527, PubMed:19221179, PubMed:20543840, PubMed:21245099). Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3' (PubMed:10358076). Activity suppressed by insulin (PubMed:10358076). Main regulator of redox balance and osteoblast numbers and controls bone mass (By similarity). Orchestrates the endocrine function of the skeleton in regulating glucose metabolism (By similarity). lso acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity (By similarity). Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP (By similarity). In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1 (By similarity). Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1 (PubMed:18356527, PubMed:19221179). Promotes neural cell death (PubMed:18356527). Mediates insulin action on adipose tissue (By similarity). Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake (By similarity). Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells (By similarity). Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner (PubMed:20543840). Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity).
Chromosomal Localization:
13q14.11
Accession Number:
NP_002006.2
UniProt Accession Number:
Q12778
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
ASLQSGQEGAGDSPGSQFSK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2200
Last Updated:
12/17/2020
Links
- EnsEMBL - EnsEMBL FOXO1
- CPTAC Assay Portal - Entrez FOXO1
- Uniprot - Uniprot Q12778
Characterization Data
SOPs
No SOPs available.
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