Western blot using CPTC-FOXC2-1 as primary antibody against FOXC2 protein domain comprising amino acids 1-70 (lane 2). Molecular weight standards are also included (lane 1).

Characterization SOP Files

Evaluation using Western Blot (108.9 KB)

Western Blot - Tissue or Cell Lysate

Click to enlarge image Western Blot using CPTC-FOXC2-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 53.7 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).

Click image to enlarge

CPTC-FOXC2-1 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-FOXC2-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 53.7 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).

Characterization SOP Files

Evaluation using Western Blot (108.9 KB)

Western Blot - Tissue or Cell Lysate

Click to enlarge image Automated Western Blot using CPTC-FOXC2-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 53.7 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).

Click image to enlarge

CPTC-FOXC2-1 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-FOXC2-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 53.7 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).

Characterization SOP Files

Protein Separation and Identification using Automated Western (267.7 KB)

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Antigen: Forkhead Box C2 Peptide 1

Background

NCI Identification Number:

00264

Antigen Name:

Forkhead Box C2 Peptide 1

CPTC Name:

CPTC-FOXC2 Peptide 1

Aliases:

Forkhead Box C2; Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1; Forkhead-Related Protein FKHL14; Mesenchyme Fork Head Protein 1; Transcription Factor FKH-14; Mesenchyme Forkhead 1; FKHL14; MFH1; Forkhead, Drosophila, Homolog-Like 14; MFH-1,Mesenchyme Forkhead 1; Forkhead Box Protein C2; MFH-1 Protein; MFH-1 ; LD

Function:

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.

FOXC2 (Forkhead Box C2) is a Protein Coding gene. Diseases associated with FOXC2 include Lymphedema-Distichiasis Syndrome and Distichiasis. Among its related pathways are Adipogenesis and Glucose / Energy Metabolism. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and transcription regulatory region DNA binding. An important paralog of this gene is FOXC1.

Transcriptional activator. Might be involved in the formation of special mesenchymal tissues.

Chromosomal Localization:

16q24.1

Accession Number:

NP_005242.1

UniProt Accession Number:

Q99958

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

VVPYLSEQNYYRAAGSYGGc

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2090

Last Updated:

09/16/2019

SOPs

No SOPs available.

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Forkhead Box C2 Peptide 1

Antibodies

CPTC-FOXC2-1

Antigen