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FBJ Murine Osteosarcoma Viral Oncogene Homolog Peptide 5

Antibody: CPTC-FOS-4
Background

Catalog Number:

CPTC-FOS-4

RRID:

AB_2722061

Target Antigen:

FBJ Murine Osteosarcoma Viral Oncogene Homolog Peptide 5

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

05/23/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Ras Pathway

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External Links
Publications
Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on normal kidney tissue core using CPTC-FOS-4 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (colon, breast, ovarian, lung, and prostate). Click image to enlarge

CPTC-FOS-4 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on normal kidney tissue core using CPTC-FOS-4 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (colon, breast, ovarian, lung, and prostate).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (310.0 KB)

CPTC-FOS-4 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Microarray core of ovarian cancer immunohistochemically stained with antibody CPTC-FOS-4 Click image to enlarge

CPTC-FOS-4 IHC

Result: Positive

Tissue Microarray core of ovarian cancer immunohistochemically stained with antibody CPTC-FOS-4


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-FOS-1 antibody with CPTC-FOS peptide 5 (NCI ID#00144) as target Click image to enlarge

CPTC-FOS-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-FOS-1 antibody with CPTC-FOS peptide 5 (NCI ID#00144) as target


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-FOS-4 antibody (green). FOS protein expression shows localization to the nucleus. Click image to enlarge

CPTC-FOS-4 Immunofluorescence

Result: Presumed Positive

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-FOS-4 antibody (green). FOS protein expression shows localization to the nucleus.


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U2OS shows localization to nucleoplasm. Human assay: THP-1 fixed with PFA, dilution: 1:100 Human assay: U2OS fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-FOS-4 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U2OS shows localization to nucleoplasm. Human assay: THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-FOS-4 as primary Ab against recombinant FOS (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-FOS-4 Western Blot

Result: Positive

Western Blot using CPTC-FOS-4 as primary Ab against recombinant FOS (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-FOS-4 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-FOS-4 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-FOS-4 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: FBJ Murine Osteosarcoma Viral Oncogene Homolog Peptide 5
Background

NCI Identification Number:

00144

Antigen Name:

FBJ Murine Osteosarcoma Viral Oncogene Homolog Peptide 5

CPTC Name:

CPTC-FOS Peptide 5

Aliases:

FBJ Murine Osteosarcoma Viral Oncogene Homolog; G0/G1 Switch Regulatory Protein 7; FBJ Murine Osteosarcoma Viral (V-Fos) Oncogene Homolog (Oncogene FOS); V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog; Cellular Oncogene C-Fos; Cellular Oncogene Fos; Activator Protein 1; C-FOS; AP-1; G0S7; P55

Function:

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

FOS (FBJ Murine Osteosarcoma Viral Oncogene Homolog) is a Protein Coding gene. Diseases associated with FOS include myositis ossificans and cerebral hypoxia. Among its related pathways are Gene Expression and IL6-mediated signaling events. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and transcription factor binding. An important paralog of this gene is FOSL1.

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

Chromosomal Localization:

14q24.3

Accession Number:

NP_005243.1

UniProt Accession Number:

P01100

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GSSSNEPSSDSLSSPTLLAL

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2200

Last Updated:

07/12/2016

Links

Characterization Data

SOPs

No SOPs available.

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