FBJ Murine Osteosarcoma Viral Oncogene Homolog Peptide 5


Background

Catalog Number:

CPTC-FOS-4

Target Antigen:

FBJ Murine Osteosarcoma Viral Oncogene Homolog Peptide 5

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Peptide, Recombinant Full-length, Native Protein

Thematic Panel(s):

Ras Pathway

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC Tissue
Click to enlarge image Tissue Microarray core of ovarian cancer immunohistochemically stained with antibody CPTC-FOS-4 Click image to enlarge

CPTC-FOS-4 IHC

Result: Positive

Tissue Microarray core of ovarian cancer immunohistochemically stained with antibody CPTC-FOS-4


  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-FOS-1 antibody with CPTC-FOS peptide 5 (NCI ID#00144) as target Click image to enlarge

CPTC-FOS-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-FOS-1 antibody with CPTC-FOS peptide 5 (NCI ID#00144) as target


  Western Blot - Cell Lysate
Click to enlarge image Western Blot using CPTC-FOS-4 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-FOS-4 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-FOS-4 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-FOS-4 as primary Ab against recombinant FOS (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-FOS-4 Western Blot

Result: Positive

Western Blot using CPTC-FOS-4 as primary Ab against recombinant FOS (lane 2). Also included are molecular wt. standards (lane 1).


Background

NCI Identification Number:

00144

Antigen Name:

FBJ Murine Osteosarcoma Viral Oncogene Homolog Peptide 5

CPTC Name:

CPTC-FOS Peptide 5

Keywords:

FBJ Murine Osteosarcoma Viral Oncogene Homolog; G0/G1 Switch Regulatory Protein 7; FBJ Murine Osteosarcoma Viral (V-Fos) Oncogene Homolog (Oncogene FOS); V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog; Cellular Oncogene C-Fos; Cellular Oncogene Fos; Activator Protein 1; C-FOS; AP-1; G0S7; P55

Function:

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

FOS (FBJ Murine Osteosarcoma Viral Oncogene Homolog) is a Protein Coding gene. Diseases associated with FOS include myositis ossificans and cerebral hypoxia. Among its related pathways are Gene Expression and IL6-mediated signaling events. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and transcription factor binding. An important paralog of this gene is FOSL1.

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

Chromosomal Localization:

14q24.3

NCBI Accession Number:

NP_005243.1

Swiss Prot Accession Number:

P01100

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GSSSNEPSSDSLSSPTLLAL

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2200

Last Updated:

07/12/2016

Links

Characterization Data

SOPs

No SOPs available.

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