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Fas Cell Surface Death Receptor Peptide 2

Antibody: CPTC-FAS-2
Background

Catalog Number:

CPTC-FAS-2

Target Antigen:

Fas Cell Surface Death Receptor Peptide 2

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

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Characterization Data [Compare Characterization Data]
  NCI 60 Protein Array

CPTC-FAS-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-FAS-2 as primary antibody against Fas (TNF receptor superfamily, member 6) (FAS) human recombinant protein, transcript variant 1 (lane 2). Expected molecular weight - 36 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-FAS-2 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-FAS-2 as primary antibody against Fas (TNF receptor superfamily, member 6) (FAS) human recombinant protein, transcript variant 1 (lane 2). Expected molecular weight - 36 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-FAS-2 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-FAS-2 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 37.7​ kDa, 11.4 kDa, ​9.4 kDa,​ 16.6 kDa, ​14.6 kDa,​ 35.4 kDa, and 24.8 kDa​. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-FAS-2 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-FAS-2 as primary antibody against buffy coat, HeLa, Jurkat, A549, MCF7 and H226 whole cell lysates. Expected molecular weight - 37.7 kDa, ​11.4 kDa, ​9.4 kDa, ​16.6 kDa, ​14.6 kDa, ​35.4 kDa and ​24.8 kDa.​ The antibody di not recognize the target in the tested cell lines.


Characterization SOP Files

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Antigen: Fas Cell Surface Death Receptor Peptide 2
Background

NCI Identification Number:

00496

Antigen Name:

Fas Cell Surface Death Receptor Peptide 2

CPTC Name:

CPTC-FAS Peptide 2

Aliases:

Fas Cell Surface Death Receptor; Tumor Necrosis Factor Receptor Superfamily, Member 6; Fas (TNF Receptor Superfamily, Member 6); Apoptosis-Mediating Surface Antigen FAS; TNF Receptor Superfamily Member 6; FASLG Receptor; CD95 Antigen; TNFRSF6; APO-1; APT1; CD95; FAS1; Mutant Tumor Necrosis Receptor Superfamily Member 6; Apoptosis Signaling Receptor FAS; APO-1 Cell Surface Antigen; Apoptosis Antigen 1; Apo-1 Antigen; Fas AMA; ALPS1A; FASTM; FAS

Function:

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.FAS (Fas Cell Surface Death Receptor) is a Protein Coding gene. Diseases associated with FAS include Autoimmune Lymphoproliferative Syndrome and Lymphoproliferative Syndrome. Among its related pathways are Apoptosis Modulation and Signaling and Signal transduction_JNK pathway. Gene Ontology (GO) annotations related to this gene include identical protein binding. An important paralog of this gene is TNFRSF10B.Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Chromosomal Localization:

10q23.31

Accession Number:

NP_000034.1

UniProt Accession Number:

P25445

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DITSDSENSNFR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

07/15/2021

Links

Characterization Data

SOPs

No SOPs available.

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