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Deleted in liver cancer 1

Antibody: CPTC-DLC1-1
Background

Catalog Number:

CPTC-DLC1-1

RRID:

AB_2617246

Target Antigen:

Deleted in liver cancer 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

01/04/2023

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (396.2 KB)

CPTC-DLC1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U2OS shows localization to cytosol. Human assay: THP-1 fixed with PFA, dilution: 1:100 Human assay: U2OS fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-DLC1-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U2OS shows localization to cytosol. Human assay: THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-DLC1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-DLC1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody Dilution 1:500 Click image to enlarge

CPTC-DLC1-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%).
Analysis performed using a standard panel of samples. Antibody Dilution 1:500


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-DLC1-1 as primary Ab against DLC1 (rAg 00016) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-DLC1-1 Western blot

Result: Positive

Western Blot using CPTC-DLC1-1 as primary Ab against DLC1 (rAg 00016) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-DLC1-2
Background

Catalog Number:

CPTC-DLC1-2

RRID:

AB_2617247

Target Antigen:

Deleted in liver cancer 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2022

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (312.0 KB)

CPTC-DLC1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-DLC1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-DLC1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-DLC1-2 as primary Ab against DLC1 (rAg 00016) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-DLC1-2 Western blot

Result: Positive

Western Blot using CPTC-DLC1-2 as primary Ab against DLC1 (rAg 00016) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-DLC1-3
Background

Catalog Number:

CPTC-DLC1-3

RRID:

AB_2617248

Target Antigen:

Deleted in liver cancer 1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2022

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (357.8 KB)

CPTC-DLC1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-DLC1-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-DLC1-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-DLC1-3 as primary Ab against DLC1 (rAg 00016) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-DLC1-3 Western blot

Result: Positive

Western Blot using CPTC-DLC1-3 as primary Ab against DLC1 (rAg 00016) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Deleted in liver cancer 1
Background

NCI Identification Number:

00016

Antigen Name:

Deleted in liver cancer 1

CPTC Name:

CPTC-DLC1

Aliases:

Deleted In Liver Cancer 1 Variant 2; ARHGAP7; STARD12; HP; P122-RhoGAP; DLC-1; Deleted In Liver Cancer 1 Protein; Rho-Type GTPase-Activating Protein 7; START Domain-Containing Protein 12; Rho GTPase-Activating Protein 7; StAR-Related Lipid Transfer (START) Domain Containing 12; KIAA1723; StARD12; HP Protein; StAR-Related Lipid Transfer Protein 12

Function:

This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.

Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality

Chromosomal Localization:

8p22

Expression System:

E. coli

Accession Number:

NM_006094

UniProt Accession Number:

Q96QB1

DNA Source:

Mammalian Gene Collection

Immunogen:

Recombinant Full Length Protein

Vector Name:

pET28-MHL

Extinction Coefficient:

Buffers:

PBS, pH 7.4

Expressed Sequence:

mhhhhhhssgrenlyfqgSVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNH
CLDQVGLFRKSGVKSRIQALRQMNEGAIDCVNYEGQSAYDVADMLKQYFR
DLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLL
YFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSL
GKPDQKDLNENLAATQGLAHMIAECKKL

Native Sequence:

Calculated Isoelectric Point:

9.1

Molecular Weight:

26004

Last Updated:

06/17/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of DLC1 (rAg 00016) with molecular weight standards in lane 1.
Click image to enlarge

CPTC-DLC1 PAGE

PAGE of DLC1 (rAg 00016) with molecular weight standards in lane 1.

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SOPs

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