PRINT

C-X-C Motif Chemokine Ligand 13 Peptide 1

Antibody: CPTC-CXCL13-1
Background

Catalog Number:

CPTC-CXCL13-1

RRID:

AB_2889961

Target Antigen:

C-X-C Motif Chemokine Ligand 13 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (358.3 KB)

CPTC-CXCL13-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CXCL13-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5936) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-CXCL13-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CXCL13-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5936)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  NCI 60 Protein Array

CTPC-CXCL13-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-CXCL13-1 Western Blot (recombinant protein)

Result: Negative

Western blot using CPTC-CXCL13-1 as primary antibody against chemokine (C-X-C motif) ligand 13 recombinant protein (lane 2). Expected molecular weight - 10.3 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-CXCL13-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-CXCL13-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight -12.7 kDa. Buffy coat and all cell lines are negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-CXCL13-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-CXCL13-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 12.7 kDa. All cell lines are negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

[top]

Antigen: C-X-C Motif Chemokine Ligand 13 Peptide 1
Background

NCI Identification Number:

00361

Antigen Name:

C-X-C Motif Chemokine Ligand 13 Peptide 1

CPTC Name:

CPTC-CXCL13 Peptide 1

Aliases:

C-X-C Motif Chemokine Ligand 13; BCA-1; BLC; Small Inducible Cytokine B Subfamily (Cys-X-Cys Motif), Member 13 (B-Cell Chemoattractant); B Cell-Attracting Chemokine 1; B Lymphocyte Chemoattractant; Small-Inducible Cytokine B13; C-X-C Motif Chemokine 13; B-Cell Chemoattractant; CXC Chemokine BLC; ANGIE2; SCYB13; ANGIE; BLR1L; BCA1; B-Cell-Homing Chemokine (Ligand For Burkitt; Chemokine (C-X-C Motif) Ligand 13 (B-Cell Chemoattractant); Chemokine (C-X-C Motif) Ligand 13; B-Cell-Attracting Chemokine 1; B-Lymphocyte Chemoattractant; CXCL13; Angie

Function:

B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. CXCL13 (C-X-C Motif Chemokine Ligand 13) is a Protein Coding gene. Diseases associated with CXCL13 include Angioimmunoblastic T-Cell Lymphoma and Asymptomatic Neurosyphilis. Among its related pathways are Signaling by GPCR and PEDF Induced Signaling. Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and chemokine activity. An important paralog of this gene is CXCL8.Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.

Chromosomal Localization:

4q21.1

Accession Number:

NP_006410.1

UniProt Accession Number:

O43927

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SIVCVDPQAEWIQR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1540

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]