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C-X-C Motif Chemokine Ligand 10 Peptide 2

Antibody: CPTC-CXCL10-2
Background

Catalog Number:

CPTC-CXCL10-2

RRID:

AB_2943348

Target Antigen:

C-X-C Motif Chemokine Ligand 10 Peptide 2

Isotype:

IgG1

Species:

Mouse Recombinant Cloned Antibody

Last Updated:

05/17/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

Characterization Data [Compare Characterization Data]
  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon showing cytoplasmic staining using Antibody CPTC-CXCL10-2. Titer: 1:1000 The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity. Click image to enlarge

CPTC-CXCL10-2 IHC Tissue

Result: Presumed Positive

Tissue Micro-Array (TMA) core of colon showing cytoplasmic staining using Antibody CPTC-CXCL10-2. Titer: 1:1000
The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CXCL10-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-CXCL10-2 as primary antibody against human chemokine (C-X-C motif) ligand 10 (CXCL10) recombinant protein (lane 2). Expected molecular weight - 8.6 kDa. Molecular weight standards are also included (lane 1). Target is shows a higher apparent M.W. than expected M.W. presumably due to potential dimerization. Click image to enlarge

CPTC-CXCL10-2 Western Blot Recombinant Protein

Result: Presumed Positive (with additional bands)

Western blot using CPTC-CXCL10-2 as primary antibody against human chemokine (C-X-C motif) ligand 10 (CXCL10) recombinant protein (lane 2). Expected molecular weight - 8.6 kDa. Molecular weight standards are also included (lane 1). Target is shows a higher apparent M.W. than expected M.W. presumably due to potential dimerization.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CXCL10-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 10.9 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CXCL10-2 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-CXCL10-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 10.9 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CXCL10-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 10.9 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CXCL10-2 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-CXCL10-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 10.9 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CXCL10-2 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 10.9 kDa. Vinculin was used as a loading control. Click image to enlarge

CPTC-CXCL10-2 Western Blot (Tissue Lysate)

Result: Negative

Western blot using CPTC-CXCL10-2 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 10.9 kDa. Vinculin was used as a loading control.


Characterization SOP Files

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Antigen: C-X-C Motif Chemokine Ligand 10 Peptide 2
Background

NCI Identification Number:

00421

Antigen Name:

C-X-C Motif Chemokine Ligand 10 Peptide 2

CPTC Name:

CPTC-CXCL10 Peptide 2

Aliases:

C-X-C Motif Chemokine Ligand 10; IP-10; Small Inducible Cytokine Subfamily B (Cys-X-Cys), Member 10; 10 KDa Interferon Gamma-Induced Protein; Small-Inducible Cytokine B10; C-X-C Motif Chemokine 10; SCYB10; GIP-10; IFI10; INP10; Crg-2; Mob-1; C7; Protein 10 From Interferon (Gamma)-Induced Cell Line; Interferon-Inducible Cytokine IP-10; Chemokine (C-X-C Motif) Ligand 10; Gamma IP10; Gamma-IP10; CXCL10

Function:

This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. CXCL10 (C-X-C Motif Chemokine Ligand 10) is a Protein Coding gene. Diseases associated with CXCL10 include Hepatitis C and Endotheliitis. Among its related pathways are Signaling by GPCR and PEDF Induced Signaling. Gene Ontology (GO) annotations related to this gene include signaling receptor binding and chemokine activity. An important paralog of this gene is CXCL9.Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (PubMed:7540647, PubMed:11157474, PubMed:22652417). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization (PubMed:12750173, PubMed:19151743). In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (PubMed:12750173, PubMed:12663757). Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).

Chromosomal Localization:

4q21.1

Accession Number:

NP_001556.2

UniProt Accession Number:

P02778

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LEIIPASQFCPR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

09/20/2020

Links

Characterization Data

SOPs

No SOPs available.

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