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C-X-C Motif Chemokine Ligand 10 Peptide 1

Antibody: CPTC-CXCL10-1
Background

Catalog Number:

CPTC-CXCL10-1

RRID:

AB_2889960

Target Antigen:

C-X-C Motif Chemokine Ligand 10 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (520.0 KB)

CPTC-CXCL10-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-CXCL10-1. Titer: 1:9000 Click image to enlarge

CTPC-CXCL10-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-CXCL10-1. Titer: 1:9000


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CXCL10-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5938) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-CXCL10-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CXCL10-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5938)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence

CPTC-CXCL10-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-CXCL10-1 Ab shows no localization of CXCL10 protein.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-CXCL10-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Click image to enlarge

CTPC-CXCL10-1 NCI60 Protein Array

Result: Positive

Protein Array in which CPTC-CXCL10-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-CXCL10-1 Western Blot (recombinant protein)

Result: Negative

Western blot using CPTC-CXCL10-1 as primary antibody against human chemokine (C-X-C motif) ligand 10 (CXCL10) recombinant protein (lane 2). Expected molecular weight - 8.6 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CXCL10-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 10.9. Buffy coat and all cell lines are negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CXCL10-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-CXCL10-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 10.9. Buffy coat and all cell lines are negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CXCL10-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 10.9 kDa. HeLa, A549, MCF7, and NCI-H226 are positive. Jurkat is negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CXCL10-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-CXCL10-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 10.9 kDa. HeLa, A549, MCF7, and NCI-H226 are positive. Jurkat is negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: C-X-C Motif Chemokine Ligand 10 Peptide 1
Background

NCI Identification Number:

00360

Antigen Name:

C-X-C Motif Chemokine Ligand 10 Peptide 1

CPTC Name:

CPTC-CXCL10 Peptide 1

Aliases:

C-X-C Motif Chemokine Ligand 10; IP-10; Small Inducible Cytokine Subfamily B (Cys-X-Cys), Member 10; 10 KDa Interferon Gamma-Induced Protein; Small-Inducible Cytokine B10; C-X-C Motif Chemokine 10; SCYB10; GIP-10; IFI10; INP10; Crg-2; Mob-1; C7; Protein 10 From Interferon (Gamma)-Induced Cell Line; Interferon-Inducible Cytokine IP-10; Chemokine (C-X-C Motif) Ligand 10; Gamma IP10; Gamma-IP10; CXCL10

Function:

This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. CXCL10 (C-X-C Motif Chemokine Ligand 10) is a Protein Coding gene. Diseases associated with CXCL10 include Hepatitis C and Endotheliitis. Among its related pathways are Signaling by GPCR and PEDF Induced Signaling. Gene Ontology (GO) annotations related to this gene include signaling receptor binding and chemokine activity. An important paralog of this gene is CXCL9.Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (PubMed:7540647, PubMed:11157474, PubMed:22652417). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization (PubMed:12750173, PubMed:19151743). In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (PubMed:12750173, PubMed:12663757). Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).

Chromosomal Localization:

4q21.1

Accession Number:

NP_001556.2

UniProt Accession Number:

P02778

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

VEIIATMK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

880

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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