Beta Catenin like protein 1


Background

Catalog Number:

CPTC-CTNNBL1-1

Target Antigen:

Beta Catenin like protein 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC NCI60

CPTC-CTNNBL1-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  IHC Tissue

CPTC-CTNNBL1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CTNNBL1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CTNNBL1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CTNNBL1-1 as primary Ab against CTNNBL1 (rAg 11057) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-CTNNBL1-1 Western Blot

Result: Positive

Western Blot using CPTC-CTNNBL1-1 as primary Ab against CTNNBL1 (rAg 11057) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

NCI Identification Number:

11057

Antigen Name:

Beta Catenin like protein 1

CPTC Name:

CPTC-CTNNBL1

Keywords:

catenin, beta like 1; NAP; C20orf33; P14L; NYD-SP19; Testis development protein NYD-SP19; Nuclear Associated Protein; FLJ21108; P14; Chromosome 20 ORF 33; PP8304; beta catenin like protein 1; dj633020.1

Function:

Induces apoptosis in CHO cells

Chromosomal Localization:

20q11.23-q12

Expression System:

E. Coli

NCBI Accession Number:

CB137946

Swiss Prot Accession Number:

Q8WYA6

DNA Source:

OpenBiosystems - MHS4426-99625720

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

19160

Buffers:

PBS without Ca++ and Mg++

Expressed Sequence:

SNAMDVGELLSYQPNRGTKRPRDDEEEEQKMRRKQTGTRERGRYREEEMT
VVEEADDDKKRLLQIIDRDGEEEEEEEEPLDESSVKKMILTFEKRSYKNQ
ELRIKFPDNPEKFMESELDLNDIIQEMHVVATMPDLYHLLVELNAVQSLL
GLLGHDNTDVSIAVVDLLQELTDIDTLHESEEGAEVLIDALVDGQVVALL
VQNLERLDESVKEEADGVHNTLAIAENMAEFRPEMCTEGAQQGLLQWLLK
RLKAKMPFDANKLYCSEVLAILLQDNDENRELLGELDGIDVLLQQLSVFK
RHNPSTAEEQEMMENLFDSLCSCLMLSSNRERFLKGEGLQLMNLMLREKK
ISRSSALKVLDHAMIGPEGTDNCHKFVDILGLRTIFPLFMKSPRKIKKVG
TTEKEHEEHVCSILASLLRNLRGQQRTRLLNKFTENDSEKVDRLMELHFK
YLGAMQVADKKIEGEKHDMVRRGEIIDNDTEEEFYLRRLDAGLFVLQHIC
YIMAEICNANVPQIRQRVHQILNMRGSSIKIVRHIIKEYAENIGDGRSPE
FRENEQKRILGLLENF

Native Sequence:

MDVGELLSYQPNRGTKRPRDDEEEEQKMRRKQTGTRERGRYREEEMTVVE
EADDDKKRLLQIIDRDGEEEEEEEEPLDESSVKKMILTFEKRSYKNQELR
IKFPDNPEKFMESELDLNDIIQEMHVVATMPDLYHLLVELNAVQSLLGLL
GHDNTDVSIAVVDLLQELTDIDTLHESEEGAEVLIDALVDGQVVALLVQN
LERLDESVKEEADGVHNTLAIAENMAEFRPEMCTEGAQQGLLQWLLKRLK
AKMPFDANKLYCSEVLAILLQDNDENRELLGELDGIDVLLQQLSVFKRHN
PSTAEEQEMMENLFDSLCSCLMLSSNRERFLKGEGLQLMNLMLREKKISR
SSALKVLDHAMIGPEGTDNCHKFVDILGLRTIFPLFMKSPRKIKKVGTTE
KEHEEHVCSILASLLRNLRGQQRTRLLNKFTENDSEKVDRLMELHFKYLG
AMQVADKKIEGEKHDMVRRGEIIDNDTEEEFYLRRLDAGLFVLQHICYIM
AEICNANVPQIRQRVHQILNMRGSSIKIVRHIIKEYAENIGDGRSPEFRE
NEQKRILGLLENF

Calculated Isoelectric Point:

4.96

Molecular Weight:

65418

Last Updated:

10/10/2019

Links

Characterization Data

Gel

Click to enlarge image PAGE of CTNNBL1 (rAg 11057) with molecular weight standards in lane 1
Click image to enlarge

CPTC-CTNNBL1 PAGE

PAGE of CTNNBL1 (rAg 11057) with molecular weight standards in lane 1

SOPs

Don't have Adobe Reader™?

Get it for free at Adobe.com