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Colony Stimulating Factor 1 Peptide 1

Antibody: CPTC-CSF1-1
Background

Catalog Number:

CPTC-CSF1-1

Target Antigen:

Colony Stimulating Factor 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (576.0 KB)

CPTC-CSF1-1 evaluation by the Human Protein Atlas

Result: Negative

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CSF1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6245) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-CSF1-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-CSF1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6245)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CSF1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate

CPTC-CSF1-1 Western Blot (Over-Expressed Lysate)

Result: Negative

Western blot using CPTC-CSF1-1 as primary antibody against colony stimulating factor 1 (CSF1) transient overexpression lysate. The expected molecular weight is 47.7 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-CSF1-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-CSF1-1 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 60.2 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-CSF1-1 Simple Western (Lysates)

Result: Negative

Automated WB (Simple Western) using CPTC-CSF1-1 as primary antibody against the whole lysate of PBMC, HeLa, Jurkat, A549, MCF7 and H226. Expected MW is 60.2 KDa. The antibody does not recognize the target in tested lysates. The same cell lysates were probed with an anti-CytC antibody.


Characterization SOP Files

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Antigen: Colony Stimulating Factor 1 Peptide 1
Background

NCI Identification Number:

00428

Antigen Name:

Colony Stimulating Factor 1 Peptide 1

CPTC Name:

CPTC-CSF1 Peptide 1

Aliases:

Colony Stimulating Factor 1; MCSF; Colony Stimulating Factor 1 (Macrophage); Macrophage Colony Stimulating Factor 1; Macrophage Colony-Stimulating Factor 1; Lanimostim; CSF-1; M-CSF; MGC31930; CSF1

Function:

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants.CSF1 (Colony Stimulating Factor 1) is a Protein Coding gene. Diseases associated with CSF1 include Pigmented Villonodular Synovitis and Tenosynovial Giant Cell Tumor. Among its related pathways are Ras signaling pathway and PEDF Induced Signaling. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and growth factor activity.Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

Chromosomal Localization:

1p13.3

Accession Number:

NP_000748.4

UniProt Accession Number:

P09603

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SHSSGSVLPLGELEGR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1760

Last Updated:

09/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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