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C-Reactive Protein Peptide 1

Antibody: CPTC-CRP-1
Background

Catalog Number:

CPTC-CRP-1

Target Antigen:

C-Reactive Protein Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-CRP-1. Titer: 1:1000 The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity. Click image to enlarge

CPTC-CRP-1 IHC Tissue

Result: Presumed Positive

Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-CRP-1. Titer: 1:1000

The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CRP-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6228) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-CRP-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-CRP-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6228)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-CRP-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-CRP-1 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-CRP-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-CRP-1 as primary antibody against human C-reactive protein, pentraxin-related (CRP) recombinant protein (lane 2). Expected molecular weight - 23 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CRP-1 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-CRP-1 as primary antibody against human C-reactive protein, pentraxin-related (CRP) recombinant protein (lane 2). Expected molecular weight - 23 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CRP-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 25.0 kDa and 10.4 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CRP-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-CRP-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 25.0 kDa and 10.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CRP-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 25.0 kDa and 10.4 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CRP-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-CRP-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 25.0 kDa and 10.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: C-Reactive Protein Peptide 1
Background

NCI Identification Number:

00495

Antigen Name:

C-Reactive Protein Peptide 1

CPTC Name:

CPTC-CRP Peptide 1

Aliases:

C-Reactive Protein; PTX1; C-Reactive Protein, Pentraxin-Related; Pentraxin 1; CRP

Function:

The protein encoded by this gene belongs to the pentraxin family which also includes serum amyloid P component protein and pentraxin 3. Pentraxins are involved in complement activation and amplification via communication with complement initiation pattern recognition molecules, but also complement regulation via recruitment of complement regulators. The encoded protein has a calcium dependent ligand binding domain with a distinctive flattened beta-jellyroll structure. It exists in two forms as either a pentamer in circulation or as a nonsoluble monomer in tissues. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. Elevated expression of the encoded protein is associated with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection.CRP (C-Reactive Protein) is a Protein Coding gene. Diseases associated with CRP include Appendicitis and Acute Pyelonephritis. Among its related pathways are Immune response Lectin induced complement pathway and Creation of C4 and C2 activators. Gene Ontology (GO) annotations related to this gene include calcium ion binding and cholesterol binding. An important paralog of this gene is APCS.Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Chromosomal Localization:

1q23.2

Accession Number:

NP_000558.2

UniProt Accession Number:

P02741

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ESDTSYVSLK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

07/15/2021

Links

Characterization Data

SOPs

No SOPs available.

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