Checkpoint Kinase 2 Peptide 1


Background

Catalog Number:

CPTC-CHEK2-1

Target Antigen:

Checkpoint Kinase 2 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/19/2020

Antigen Recognition(s):

Peptide, Phosphorylation

Background

NCI Identification Number:

00303

Antigen Name:

Checkpoint Kinase 2 Peptide 1

CPTC Name:

CPTC-CHEK2 Peptide 1

Keywords:

Checkpoint Kinase 2; Serine/Threonine-Protein Kinase Chk2; CHK2 Checkpoint Homolog; Cds1 Homolog; RAD53; HCds1; CDS1; CHK2; CHK2 (Checkpoint, S.Pombe) Homolog; CHK2 Checkpoint Homolog (S. Pombe); Checkpoint-Like Protein CHK2; EC 2.7.11.1; EC 2.7.11; HuCds1; PP1425; Hucds1; LFS2

Function:

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. CHEK2 (Checkpoint Kinase 2) is a Protein Coding gene. Diseases associated with CHEK2 include Prostate Cancer and Li-Fraumeni Syndrome 2. Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and Human T-cell leukemia virus 1 infection. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and transferase activity, transferring phosphorus-containing groups.Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition.Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. Two subtypes have so far been identified, Chk1 and Chk2. They are essential components to delay cell cycle progression in all cells and act at all three cell cycle checkpoints.

Chromosomal Localization:

22q12.1

NCBI Accession Number:

na

UniProt Accession Number:

O96017

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CILGETS(ph)LM(ox)R

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

10/23/2019

Links

Characterization Data

SOPs

No SOPs available.

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