Cyclin Dependent Kinase 1 Peptide 1


Background

Catalog Number:

CPTC-CDK1-1

RRID:

AB_2877655

Target Antigen:

Cyclin Dependent Kinase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

11/16/2021

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (477.3 KB)

CPTC-CDK1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  IHC Tissue

CTPC-CDK1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  Immunofluorescence
Click to enlarge image Immunofluorescence staining of human cell line LCL57 with CPTC-CDK1-1 Ab shows localization to the mitochondrion, nucleus, cytoskeleton, other. Click image to enlarge

CPTC-CDK1-1

Result: Positive

Immunofluorescence staining of human cell line LCL57 with CPTC-CDK1-1 Ab shows localization to the mitochondrion, nucleus, cytoskeleton, other.


  Western Blot - Cell Lysate
Click to enlarge image Western Blot using CPTC-CDK1-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1) Click image to enlarge

CPTC-CDK1-1 western blot (cell lysates)

Result: Positive

Western Blot using CPTC-CDK1-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1)


Characterization SOP Files

  Western Blot - Cell Lysate
Click to enlarge image Automated western blot using CPTC-CDK1-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1) Click image to enlarge

CPTC-CDK1-1 automated western blot (cell lysate)

Result: Positive

Automated western blot using CPTC-CDK1-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1)


  Western Blot - Cell Lysate
Click to enlarge image Single cell western blot using CPTC-CDK1-1 as primary antibody against whole cell lysates. Relative expression of total CDK1 (34 kDa band) in HeLa, MCF10A, and LCL57 cells (A). Percentage of cells that express CDK1 (B). Average expression of CDK1 protein per cell (C). All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-CDK1-1 Single Cell Western Blot Cell Lysate

Result: Positive

Single cell western blot using CPTC-CDK1-1 as primary antibody against whole cell lysates. Relative expression of total CDK1 (34 kDa band) in HeLa, MCF10A, and LCL57 cells (A). Percentage of cells that express CDK1 (B). Average expression of CDK1 protein per cell (C). All data is normalized to β-tubulin expression.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CDK1-1 as primary antibody against CDK1 recombinant protein (lane 2). Also included are molecular weight standard (lane 1) Click image to enlarge

CPTC-CDK1-1 western blot (recombinant protein)

Result: Positive

Western Blot using CPTC-CDK1-1 as primary antibody against CDK1 recombinant protein (lane 2). Also included are molecular weight standard (lane 1)


Characterization SOP Files

Background

NCI Identification Number:

00311

Antigen Name:

Cyclin Dependent Kinase 1 Peptide 1

CPTC Name:

CPTC-CDK1 Peptide 1

Aliases:

Cyclin Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; Cyclin-Dependent Kinase 1; P34 Protein Kinase; P34CDC2; CDC28A; CDC2; Cell Cycle Controller CDC2; EC 2.7.11.22; EC 2.7.11.23; CDKN1

Function:

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.CDK1 (Cyclin Dependent Kinase 1) is a Protein Coding gene. Diseases associated with CDK1 include Retinoblastoma and Spondylocarpotarsal Synostosis Syndrome. Among its related pathways are Human cytomegalovirus infection and Oocyte meiosis. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CDK2.Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CENPA, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SAMHD1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis (PubMed:26549230). Regulates the amplitude of the cyclic expression of the core clock gene ARNTL/BMAL1 by phosphorylating its transcriptional repressor NR1D1, and this phosphorylation is necessary for SCF(FBXW7)-mediated ubiquitination and proteasomal degradation of NR1D1.

Chromosomal Localization:

10q21.2

Accession Number:

NP_001777.1

UniProt Accession Number:

P06493

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CVYT(ph)HEVVTLWYR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1430

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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