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Cadherin 2 Peptide 5

Antibody: CPTC-CDH2-4
Background

Catalog Number:

CPTC-CDH2-4

RRID:

AB_2722044

Target Antigen:

Cadherin 2 Peptide 5

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (322.3 KB)

CPTC-CDH2-4 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CDH2-4 antibody with CPTC-CDH2 peptide 5 (NCI ID#176) as target Click image to enlarge

CPTC-CDH2-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CDH2-4 antibody with CPTC-CDH2 peptide 5 (NCI ID#176) as target


Characterization SOP Files

  Immunofluorescence

CPTC-CDH2-4 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC- CDH2-4 antibody. CDH2 protein expression was not detected.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CDH2-4 as primary Ab against recombinant CDH2 (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-CDH2-4 Western Blot

Result: Positive

Western Blot using CPTC-CDH2-4 as primary Ab against recombinant CDH2 (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-CDH2-4 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-CDH2-4 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-CDH2-4 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antibody: CPTC-CDH2-5
Background

Catalog Number:

CPTC-CDH2-5

RRID:

AB_2722045

Target Antigen:

Cadherin 2 Peptide 5

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

10/04/2024

Antigen Recognition(s):

Peptide

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (305.1 KB)

CPTC-CDH2-5 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CDH2-5 antibody with CPTC-CDH2 peptide 5 (NCI ID#176) as target Click image to enlarge

CPTC-CDH2-5 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CDH2-5 antibody with CPTC-CDH2 peptide 5 (NCI ID#176) as target


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CDH2-5 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

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Antigen: Cadherin 2 Peptide 5
Background

NCI Identification Number:

00176

Antigen Name:

Cadherin 2 Peptide 5

CPTC Name:

CPTC-CDH2 Peptide 5

Aliases:

Cadherin 2; Cadherin 2, Type 1, N-Cadherin (Neuronal); Neural Cadherin; N-Cadherin; CDw325; NCAD; CDHN; Calcium-Dependent Adhesion Protein, Neuronal; CD325 Antigen; N-Cadherin 1; Cadherin-2; CD325

Function:

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.

CDH2 (Cadherin 2) is a Protein Coding gene. Diseases associated with CDH2 include Arrhythmogenic Right Ventricular Cardiomyopathy and Pseudomyxoma Peritonei. Among its related pathways are Blood-Brain Barrier Pathway: Anatomy and WNT Signaling. GO annotations related to this gene include calcium ion binding and protein phosphatase binding. An important paralog of this gene is CDH4.

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

Chromosomal Localization:

18q12.1

Accession Number:

XP_016881003.1

UniProt Accession Number:

P19022

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SAAPHPGDIGDFINEGLK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1980

Last Updated:

10/01/2019

Links

Characterization Data

SOPs

No SOPs available.

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