Background
Catalog Number:
CPTC-CDH1-3
RRID:
AB_2722039
Target Antigen:
Cadherin 1 Peptide 5
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/22/2022
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-CDH1-3
Publications
- A dataset describing a suite of novel antibody reagents for the RAS signaling network (PMID: 31467290)
- Targeted mass-spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP kinase, and AKT signaling (PMID: 34671754)
Characterization Data [Compare Characterization Data]
IHC Tissue
CPTC-CDH1-3 IHC
Result: Positive
Tissue Microarray core of ovarian cancer showing immunohistochemical staining using antibody CPTC-CDH1-3.
Characterization SOP Files
Immuno-MRM
CPTC-CDH1-3 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-CDH1-3 antibody with CPTC-CDH1 peptide 5 (NCI ID#171) as target
Western Blot - Recombinant Protein or Peptide
CPTC-CDH1-3 Western Blot
Result: Positive
Western Blot using CPTC-CDH1-3 as primary Ab against recombinant CDH1 (lane 2). Also included are molecular wt. standards (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CDH1-3 Cell Lysate Western Blot
Result: Positive
Western Blot using CPTC-CDH1-3 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1).
Characterization SOP Files
Background
Catalog Number:
CPTC-CDH1-4
RRID:
AB_2722040
Target Antigen:
Cadherin 1 Peptide 5
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
11/13/2019
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-CDH1-4
Publications
Characterization Data [Compare Characterization Data]
IHC Tissue
CPTC-CDH1-4 IHC
Result: Positive
Tissue Microarray cor of breast cancer showing immunohistochemical staining for CPTC-CDH1-4.
Characterization SOP Files
Immuno-MRM
CPTC-CDH1-4 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-CDH1-4 antibody with CPTC-CDH1 peptide 5 (NCI ID#171) as target
Western Blot - Recombinant Protein or Peptide
CPTC-CDH1-4 Western Blot
Result: Positive
Western Blot using CPTC-CDH1-4 as primary Ab against CDH1 (lane 2). Also included are molecular wt. standards (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CDH1-4 Cell Lysate Western Blot
Result: Positive
Western Blot using CPTC-CDH1-4 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00171
Antigen Name:
Cadherin 1 Peptide 5
CPTC Name:
CPTC-CDH1 Peptide 5
Aliases:
Cadherin 1; E-Cadherin; Cadherin 1, Type 1, E-Cadherin (Epithelial); Epithelial Cadherin; CAM 120/80; Uvomorulin; CDHE; UVO; Calcium-Dependent Adhesion Protein, Epithelial; Cadherin 1, E-Cadherin (Epithelial); Cadherin 1, Type 1; Cell-CAM 120/80; CD324 Antigen; E-Cadherin 1; Cadherin-1; Arc-1; CD324; ECAD; LCAM
Function:
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.
CDH1 (Cadherin 1) is a Protein Coding gene. Diseases associated with CDH1 include Gastric Cancer Risk After H. Pylori Infection and Endometrial Cancer. Among its related pathways are Pathways in cancer and Nectin adhesion pathway. GO annotations related to this gene include calcium ion binding and protein phosphatase binding. An important paralog of this gene is CDH3.
adherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
Chromosomal Localization:
16q22.1
Accession Number:
XP_011521791.1
UniProt Accession Number:
P12830
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
GQVPENEANVVITTLK
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1760
Last Updated:
10/01/2019
Links
- EnsEMBL - EnsEMBL CDH1
- Entrez - Entrez CDH1
- Uniprot - Uniprot P12830
Characterization Data
SOPs
No SOPs available.
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