Background
Catalog Number:
CPTC-CDCA8-1
Target Antigen:
Cell Division Cycle Associated 8 Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
03/20/2024
Antigen Recognition(s):
Peptide, Endogenous, Phosphorylation
External Links
- CPTAC Assay Portal CPTAC-5921 (Phospho)
Purchase
- DSHB CPTC-CDCA8-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (302.9 KB)
CPTC-CDCA8-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC Tissue
CTPC-CDCA8-1 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Immuno-MRM
CPTC-CDCA8-1 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-CDCA8-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5921)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CDCA8-1 automated western blot (cell lysate)
Result: Negative
Automated western blot using CPTC-CDCA8-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line.
Characterization SOP Files
Background
NCI Identification Number:
00295
Antigen Name:
Cell Division Cycle Associated 8 Peptide 1
CPTC Name:
CPTC-CDCA8 Peptide 1
Aliases:
Cell Division Cycle Associated 8; Borealin; Pluripotent Embryonic Stem Cell-Related Gene 3 Protein; Cell Division Cycle-Associated Protein 8; HDasra-B; Dasra-B; BOREALIN; Dasra B; PESCRG3; DasraB; MESRGP; BOR
Function:
This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16.CDCA8 (Cell Division Cycle Associated 8) is a Protein Coding gene. Among its related pathways are Signaling by Rho GTPases and Mitotic Metaphase and Anaphase.Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
Chromosomal Localization:
1p34.3
Accession Number:
NP_001243804.1
UniProt Accession Number:
Q53HL2
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
CLTAEAIQT(ph)PLK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1320
Last Updated:
08/21/2020
Links
- EnsEMBL - EnsEMBL CDCA8
- Entrez - Entrez CDCA8
- Uniprot - Uniprot Q53HL2
Characterization Data
SOPs
No SOPs available.
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