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Cell Division Cycle 25B Peptide 2

Antibody: CPTC-CDC25B-2
Background

Catalog Number:

CPTC-CDC25B-2

Target Antigen:

Cell Division Cycle 25B Peptide 2

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

04/19/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (510.8 KB)

CPTC-CDC25B-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue

CTPC-CDC25B-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CDC25B-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5904 for non-phosphorylated peptide and https://assays.cancer.gov/CPTAC-590 for phosphorylated peptide) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-CDC25B-2 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CDC25B-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5904 for non-phosphorylated peptide and https://assays.cancer.gov/CPTAC-590 for phosphorylated peptide)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CDC25B-2 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line. Click image to enlarge

CPTC-CDC25B-2 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-CDC25B-2 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line.


Characterization SOP Files

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Antigen: Cell Division Cycle 25B Peptide 2
Background

NCI Identification Number:

00309

Antigen Name:

Cell Division Cycle 25B Peptide 2

CPTC Name:

CPTC-CDC25B Peptide 2

Aliases:

Cell Division Cycle 25B; Dual Specificity Phosphatase Cdc25B; M-Phase Inducer Phosphatase 2; EC 3.1.3.48; CDC25HU2

Function:

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. CDC25B (Cell Division Cycle 25B) is a Protein Coding gene. Diseases associated with CDC25B include Breast Cancer and Esophageal Cancer. Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and Cell Cycle, Mitotic. Gene Ontology (GO) annotations related to this gene include protein kinase binding and protein tyrosine phosphatase activity. An important paralog of this gene is CDC25A.Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. Cdc25 is a dual specificity phosphatase with three isoforms in mammalian cells - Cdc25A, B and C. Cdc25 activates cdk complexes that drive the cell cycle. Cdc25 is involved in the DNA damage checkpoints and is known as a key mediator of cell cycle progression.

Chromosomal Localization:

20p13

Accession Number:

NP_068659.1

UniProt Accession Number:

P30305

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CSPS(ph)MPC(Cam)SVIRPILK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1650

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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