CD8A Molecule Peptide 1


Background

Catalog Number:

CPTC-CD8A-1

RRID:

AB_2890079

Target Antigen:

CD8A Molecule Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

06/04/2024

Antigen Recognition(s):

Peptide

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Characterization Data [Compare Characterization Data]
  NCI 60 Protein Array

CPTC-CD8A-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Over-expressed transient protein in cell lysate

CPTC-CD8A-1 Western Blot (Over-Expressed Lysate)

Result: Negative

Western blot using CPTC-CD8A-1 as primary antibody against transient CD8A overexpression lysate. The expected molecular weight is 25.7 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD8A-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates.  Expected molecular weight - 25.7 kDa, 21.6 kDa, and 30.2 kDa.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD8A-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-CD8A-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 25.7 kDa, 21.6 kDa, and 30.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD8A-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates.  Expected molecular weight - 25.7 kDa,​ 21.6 kDa, and ​30.2 kDa.​ Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD8A-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-CD8A-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 25.7 kDa,​ 21.6 kDa, and ​30.2 kDa.​ Molecular weight standards are also included (lane 1).


  Western Blot - Tissue or Cell Lysate

CPTC-CD8A-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-CD8A-1 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 25.7 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

Background

NCI Identification Number:

00359

Antigen Name:

CD8A Molecule Peptide 1

CPTC Name:

CPTC-CD8A Peptide 1

Aliases:

CD8a Molecule; T-Cell Surface Glycoprotein CD8 Alpha Chain; T-Lymphocyte Differentiation Antigen T8/Leu-2; CD8 Antigen, Alpha Polypeptide (P32); Leu2 T-Lymphocyte Antigen; T-Cell Antigen Leu2; T Cell Co-Receptor; T8 T-Cell Antigen; CD8a Antigen; Leu2; CD8A; CD8; P32; MAL

Function:

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. CD8A (CD8a Molecule) is a Protein Coding gene. Diseases associated with CD8A include Cd8 Deficiency, Familial and Herpes Zoster. Among its related pathways are TCR Signaling (Qiagen) and T cell receptor signaling pathway. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and coreceptor activity.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Chromosomal Localization:

2p11.2

Accession Number:

NP_001369627.1

UniProt Accession Number:

P01732

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

TWNLGETVELK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

09/17/2020

Links

Characterization Data

SOPs

No SOPs available.

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