PRINT

CD48 Molecule Peptide 1

Antibody: CPTC-CD48-1
Background

Catalog Number:

CPTC-CD48-1

Target Antigen:

CD48 Molecule Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (518.3 KB)

CPTC-CD48-1 evaluation by the Human Protein Atlas

Result: Negative

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue

CPTC-CD48-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CD48-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-CD48-1 as primary antibody against human CD48 recombinant protein, mFc-His Tag (lane 2). Expected molecular weight - 69 - 72 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD48-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-CD48-1 as primary antibody against human CD48 recombinant protein, mFc-His Tag (lane 2). Expected molecular weight - 69 - 72 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD48-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 28 kDa, 19 kDa, and 69 - 72 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD48-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-CD48-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 28 kDa, 19 kDa, and 69 - 72 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD48-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 27.7 kDa and 19.4 kDa. Molecular weight standards are also included (lane 1). Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

CPTC-CD48-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-CD48-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 27.7 kDa and 19.4 kDa. Molecular weight standards are also included (lane 1). Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD48-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 27.9 kDa. Vinculin was used as a loading control. Click image to enlarge

CPTC-CD48-1 Western Blot (Tissue Lysate)

Result: Negative

Western blot using CPTC-CD48-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 27.9 kDa. Vinculin was used as a loading control.


Characterization SOP Files

[top]

Antigen: CD48 Molecule Peptide 1
Background

NCI Identification Number:

00427

Antigen Name:

CD48 Molecule Peptide 1

CPTC Name:

CPTC-CD48 Peptide 1

Aliases:

CD48 Molecule; SLAMF2; Signaling Lymphocytic Activation Molecule 2; CD48 Antigen (B-Cell Membrane Protein); B-Lymphocyte Activation Marker BLAST-1; Leukocyte Antigen MEM-102; BCM1 Surface Antigen; SLAM Family Member 2; CD48 Antigen; BLAST1; BLAST; HCD48; MCD48; TCT.1; BCM1; MEM-102; CD48

Function:

This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.CD48 (CD48 Molecule) is a Protein Coding gene. Diseases associated with CD48 include Lymphoproliferative Syndrome, X-Linked, 1 and Cone-Rod Dystrophy 6. Among its related pathways are Gastric Cancer Network 2 and Cell surface interactions at the vascular wall. Gene Ontology (GO) annotations related to this gene include antigen binding. An important paralog of this gene is SLAMF1.Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

Chromosomal Localization:

1q23.3

Accession Number:

NP_001769.2

UniProt Accession Number:

P09326

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LQVLDPVPKPVIK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1430

Last Updated:

09/21/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]