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CD47 Molecule Peptide 1

Antibody: CPTC-CD47-4
Background

Catalog Number:

CPTC-CD47-4

RRID:

AB_2889958

Target Antigen:

CD47 Molecule Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (306.8 KB)

CPTC-CD47-4 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-CD47-4 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-CD47-4 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CD47-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5975) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-CD47-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CD47-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5975)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence

CPTC-CD47-4 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-CD47-4 Ab shows no localization of CD47 protein.


Characterization SOP Files

  NCI 60 Protein Array

CTPC-CD47-4 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-CD47-4 Western Blot (recombinant protein)

Result: Negative

Western blot using CPTC-CD47-4 as primary antibody against human CD47 recombinant protein (lane 2). Expected molecular weight - 35.21 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD47-4 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 35.2 kDa, 31.7 kDa, 33.2 kDa, and 33.8 kDa. Buffy coat is positive. All cell lines are negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD47-4 Automated Western Blot (Cell Lysate)

Result: Positive

Automated western blot using CPTC-CD47-4 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 35.2 kDa, 31.7 kDa, 33.2 kDa, and 33.8 kDa. Buffy coat is positive. All cell lines are negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD47-4 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 35.2 kDa, 31.7 kDa, 33.2 kDa, and 33.8 kDa. All cell lines are positive. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD47-4 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-CD47-4 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 35.2 kDa, 31.7 kDa, 33.2 kDa, and 33.8 kDa. All cell lines are positive. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: CD47 Molecule Peptide 1
Background

NCI Identification Number:

00357

Antigen Name:

CD47 Molecule Peptide 1

CPTC Name:

CPTC-CD47 Peptide 1

Aliases:

CD47 Molecule; Antigenic Surface Determinant Protein OA3; Leukocyte Surface Antigen CD47 2 3 4 IAP; CD47 Antigen (Rh-Related Antigen, Integrin-Associated Signal Transducer); Antigen Identified By Monoclonal Antibody 1D8; Integrin Associated Protein; Rh-Related Antigen; CD47 Glycoprotein; MER6; OA3; Integrin-Associated Signal Transducer; Integrin-Associated Protein; Protein MER6; CD47 Antigen; CD47

Function:

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.CD47 (CD47 Molecule) is a Protein Coding gene. Diseases associated with CD47 include Hereditary Spherocytosis and Neonatal Meningitis. Among its related pathways are Integrin Pathway and Integrins in angiogenesis. Gene Ontology (GO) annotations related to this gene include thrombospondin receptor activity.Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Chromosomal Localization:

3q13.12

Accession Number:

NP_001768.1

UniProt Accession Number:

Q08722

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

STVPTDFSSAK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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