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CD44 molecule (Indian blood group) Peptide 2

Antibody: CPTC-CD44-2
Background

Catalog Number:

CPTC-CD44-2

RRID:

AB_2889957

Target Antigen:

CD44 molecule (Indian blood group) Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (382.0 KB)

CPTC-CD44-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CD44-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6214) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-CD44-2 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-CD44-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6214)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array

CTPC-CD44-2 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-CD44-2 Western Blot (recombinant protein)

Result: Negative

Western blot using CPTC-CD44-2 as primary antibody against human CD44 molecule (Indian blood group) (CD44), transcript variant 1 recombinant protein (lane 2). Expected molecular weight - 23.1 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD44-2 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 81.5 kDa. More than 19 isoforms of CD44 have been identified. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD44-2 Western Blot (Cell Lysate)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-CD44-2 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 81.5 kDa. More than 19 isoforms of CD44 have been identified. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD44-2 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. . Molecular weight standards are also included (lane 1). All tested cell lines are negative. Click image to enlarge

CPTC-CD44-2 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-CD44-2 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. . Molecular weight standards are also included (lane 1). All tested cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD44-2 as primary antibody against human breast (2), ovary (3), spleen (4), endometrium (5), and lung (6) tissue lysates. The expected molecular weight is 81.5 kDa. Cytochrome C was used as a loading control. Breast and endometrium tissues are presumed positive. All other tissues are negative. Click image to enlarge

CPTC-CD44-2 Western Blot (Tissue Lysates)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-CD44-2 as primary antibody against human breast (2), ovary (3), spleen (4), endometrium (5), and lung (6) tissue lysates. The expected molecular weight is 81.5 kDa. Cytochrome C was used as a loading control. Breast and endometrium tissues are presumed positive. All other tissues are negative.


Characterization SOP Files

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Antigen: CD44 molecule (Indian blood group) Peptide 2
Background

NCI Identification Number:

00356

Antigen Name:

CD44 molecule (Indian blood group) Peptide 2

CPTC Name:

CPTC-CD44 Peptide 2

Aliases:

CD44 molecule (Indian blood group) Peptide 1; Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein 1; Hyaluronate Receptor; Hermes Antigen; CD44 Antigen; ECMR-III; HUTCH-I; Epican; CSPG8; HCELL; MC56; MDU2; MDU3; MIC4; Pgp1; LHR; IN; CD44 Antigen (Homing Function And Indian Blood Group System); Homing Function And Indian Blood Group System; Cell Surface Glycoprotein CD44; Indian Blood Group Antigen; Phagocytic Glycoprotein I; Soluble CD44; CDW44; CD44R; CDw44; PGP-1; CD44

Function:

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. CD44 (CD44 Molecule (Indian Blood Group)) is a Protein Coding gene. Diseases associated with CD44 include Superficial Keratitis and Intraductal Papilloma. Among its related pathways are Degradation of the extracellular matrix and Cell surface interactions at the vascular wall. Gene Ontology (GO) annotations related to this gene include transmembrane signaling receptor activity and cytokine receptor activity. An important paralog of this gene is LYVE1.Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).

Chromosomal Localization:

11p13

Accession Number:

NP_000601.3

UniProt Accession Number:

P16070

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

YGFIEGHVVIPR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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