Background
Catalog Number:
CPTC-CD40-2
Target Antigen:
CD40 Molecule Peptide 2
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
12/06/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-CD40-2
Characterization Data [Compare Characterization Data]
NCI 60 Protein Array
CPTC-CD40-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Background
Catalog Number:
CPTC-CD40-3
Target Antigen:
CD40 Molecule Peptide 2
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
10/18/2023
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-CD40-4
Target Antigen:
CD40 Molecule Peptide 2
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
03/20/2024
Antigen Recognition(s):
Peptide, Endogenous
Thematic Panel(s):
Immuno-Oncology (IO)
External Links
- CPTAC Assay Portal CPTAC-5957
Publications
Characterization Data [Compare Characterization Data]
Immuno-MRM
CPTC-CD40-4 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-CD40-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5957)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.
Characterization SOP Files
NCI 60 Protein Array
CPTC-CD40-4 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CD40-4 Western Blot Recombinant Protein
Result: Negative
Western blot using CPTC-CD40-4 as primary antibody against human CD40 Protein, mFc-His Tag recombinant protein (lane 2). Expected molecular weight - 68 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CD40-4 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-CD40-4 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 30.6 kDa and 22.3 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CD40-4 Automated Western Blot Cell Lysate
Result: Positive
Automated western blot using CPTC-CD40-4 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 30.6 kDa and 22.3 kDa. Molecular weight standards are also included (lane 1). HeLa is positive. A549, MCF7, and H226 are presumed positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.
Characterization SOP Files
Background
NCI Identification Number:
00469
Antigen Name:
CD40 Molecule Peptide 2
CPTC Name:
CPTC-CD40 Peptide 2
Aliases:
CD40 Molecule; Bp50; Tumor Necrosis Factor Receptor Superfamily Member 5; CD40 Molecule, TNF Receptor Superfamily Member 5; CD40L Receptor; TNFRSF5; P50; Tumor Necrosis Factor Receptor Superfamily, Member 5; B Cell Surface Antigen CD40; B-Cell Surface Antigen CD40; B Cell-Associated Molecule; CD40 Antigen; CDW40; CDw40; CD40
Function:
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. CD40 (CD40 Molecule) is a Protein Coding gene. Diseases associated with CD40 include Immunodeficiency With Hyper-Igm, Type 3 and Immunodeficiency With Hyper-Igm, Type 1. Among its related pathways are Transcriptional misregulation in cancer and NF-KappaB Family Pathway. Gene Ontology (GO) annotations related to this gene include enzyme binding. An important paralog of this gene is TNFRSF11A.Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Chromosomal Localization:
20q13.12
Accession Number:
NP_001241.1
UniProt Accession Number:
P25942
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
SCSPGFGVK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
990
Last Updated:
12/16/2020
Links
- EnsEMBL - EnsEMBL CD40
- Entrez - Entrez CD40
- Uniprot - Uniprot P25942
Characterization Data
SOPs
No SOPs available.
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