CD40 Molecule Peptide 2


Background

Catalog Number:

CPTC-CD40-2

Target Antigen:

CD40 Molecule Peptide 2

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

02/28/2024

Antigen Recognition(s):

Peptide

Purchase
Characterization Data [Compare Characterization Data]
  NCI 60 Protein Array

CPTC-CD40-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Background

Catalog Number:

CPTC-CD40-3

Target Antigen:

CD40 Molecule Peptide 2

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

10/18/2023

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
Background

Catalog Number:

CPTC-CD40-4

Target Antigen:

CD40 Molecule Peptide 2

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Publications
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CD40-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5957)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-CD40-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CD40-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5957)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


  NCI 60 Protein Array

CPTC-CD40-4 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide

CPTC-CD40-4 Western Blot Recombinant Protein

Result: Negative

Western blot using CPTC-CD40-4 as primary antibody against human CD40 Protein, mFc-His Tag recombinant protein (lane 2). Expected molecular weight - 68 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD40-4 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates.  Expected molecular weight - 30.6 kDa and 22.3 kDa.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD40-4 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-CD40-4 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 30.6 kDa and 22.3 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD40-4 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates.  Expected molecular weight - 30.6 kDa and 22.3 kDa.  Molecular weight standards are also included (lane 1). HeLa is positive. A549, MCF7, and H226 are presumed positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

CPTC-CD40-4 Automated Western Blot Cell Lysate

Result: Positive

Automated western blot using CPTC-CD40-4 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 30.6 kDa and 22.3 kDa. Molecular weight standards are also included (lane 1). HeLa is positive. A549, MCF7, and H226 are presumed positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.


Background

NCI Identification Number:

00469

Antigen Name:

CD40 Molecule Peptide 2

CPTC Name:

CPTC-CD40 Peptide 2

Aliases:

CD40 Molecule; Bp50; Tumor Necrosis Factor Receptor Superfamily Member 5; CD40 Molecule, TNF Receptor Superfamily Member 5; CD40L Receptor; TNFRSF5; P50; Tumor Necrosis Factor Receptor Superfamily, Member 5; B Cell Surface Antigen CD40; B-Cell Surface Antigen CD40; B Cell-Associated Molecule; CD40 Antigen; CDW40; CDw40; CD40

Function:

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. CD40 (CD40 Molecule) is a Protein Coding gene. Diseases associated with CD40 include Immunodeficiency With Hyper-Igm, Type 3 and Immunodeficiency With Hyper-Igm, Type 1. Among its related pathways are Transcriptional misregulation in cancer and NF-KappaB Family Pathway. Gene Ontology (GO) annotations related to this gene include enzyme binding. An important paralog of this gene is TNFRSF11A.Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).

Chromosomal Localization:

20q13.12

Accession Number:

NP_001241.1

UniProt Accession Number:

P25942

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SCSPGFGVK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

990

Last Updated:

12/16/2020

Links

Characterization Data

SOPs

No SOPs available.

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