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CD40 Molecule Peptide 1

Antibody: CPTC-CD40-1
Background

Catalog Number:

CPTC-CD40-1

RRID:

AB_2889956

Target Antigen:

CD40 Molecule Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (376.8 KB)

CPTC-CD40-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-CD40-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-CD40-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CD40-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5956) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-CD40-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CD40-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5956)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence

CPTC-CD40-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-CD40-1 Ab shows no localization of CD40 protein.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CD40-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-CD40-1 Western Blot (recombinant protein)

Result: Negative

Western blot using CPTC-CD40-1 as primary antibody against human CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 1 recombinant protein (lane 2). Expected molecular weight - 20.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD40-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 29.3. Buffy coat is positive. All cell lines are negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD40-1 Automated Western Blot (Cell Lysate)

Result: Positive

Automated western blot using CPTC-CD40-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 29.3. Buffy coat is positive. All cell lines are negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD40-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 29.3. All cell lines are positive. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD40-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-CD40-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 29.3. All cell lines are positive. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: CD40 Molecule Peptide 1
Background

NCI Identification Number:

00355

Antigen Name:

CD40 Molecule Peptide 1

CPTC Name:

CPTC-CD40 Peptide 1

Aliases:

CD40 Molecule; Bp50; Tumor Necrosis Factor Receptor Superfamily Member 5; CD40 Molecule, TNF Receptor Superfamily Member 5; CD40L Receptor; TNFRSF5; P50; Tumor Necrosis Factor Receptor Superfamily, Member 5; B Cell Surface Antigen CD40; B-Cell Surface Antigen CD40; B Cell-Associated Molecule; CD40 Antigen; CDW40; CDw40; CD40

Function:

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. CD40 (CD40 Molecule) is a Protein Coding gene. Diseases associated with CD40 include Immunodeficiency With Hyper-Igm, Type 3 and Immunodeficiency With Hyper-Igm, Type 1. Among its related pathways are Transcriptional misregulation in cancer and NF-KappaB Family Pathway. Gene Ontology (GO) annotations related to this gene include enzyme binding. An important paralog of this gene is TNFRSF11A.Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).

Chromosomal Localization:

20q13.12

Accession Number:

NP_001241.1

UniProt Accession Number:

P25942

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

YCDPNLGLR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

990

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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