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CD274 Molecule Peptide 1

Antibody: CPTC-CD274-1
Background

Catalog Number:

CPTC-CD274-1

RRID:

AB_2889053

Target Antigen:

CD274 Molecule Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

12/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (228.8 KB)

CPTC-CD274-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue

CTPC-CD274-1 IHC Tissue

Result: Negative

Tissue Micro-Array(TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-CD274-1. Titer: 1:1000


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CD274-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5988) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-CD274-1

Result: Positive

Immuno-MRM chromatogram of CPTC-CD274-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5988)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence

CPTC-CD274-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-CD274-1 Ab shows no localization of CD274 protein.


Characterization SOP Files

  NCI 60 Protein Array

CTPC-CD274-1 NCI60 Protein Array

Result: Negative

Protein Array in which CPTC-CD274-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-CD274-1 as primary antibody against human PD-L1 / CD274 recombinant protein (lane 2). Expected molecular weight - 26.33 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD274-1 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-CD274-1 as primary antibody against human PD-L1 / CD274 recombinant protein (lane 2). Expected molecular weight - 26.33 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD274-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 26.33 kDa. Molecular weight standards are also included (lane 1). Inconclusive data. Click image to enlarge

CPTC-CD274-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-CD274-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 26.33 kDa. Molecular weight standards are also included (lane 1). Inconclusive data.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD274-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 33.3 kDa, 20.2 kDa, and 20.5 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Jurkat and MCF-7 are presumed positive. All other cell lines are negative. Click image to enlarge

CPTC-CD274-1 Western Blot (Cell Lysate)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-CD274-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 33.3 kDa, 20.2 kDa, and 20.5 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Jurkat and MCF-7 are presumed positive. All other cell lines are negative.


Characterization SOP Files

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Antigen: CD274 Molecule Peptide 1
Background

NCI Identification Number:

00351

Antigen Name:

CD274 Molecule Peptide 1

CPTC Name:

CPTC-CD274 Peptide 1

Aliases:

CD274 Molecule; Programmed Cell Death 1 Ligand 1; CD274 Antigen; B7 Homolog 1; PD-L1; B7H1; PDL1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1; HPD-L1; B7-H1; B7-H; Programmed Death Ligand 1; CD274

Function:

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. CD274, also commonly referred to as PDL1, is a ligand that binds with the receptor PD1, commonly found on T-cells, and acts to block T-cell activation. PD1 expression has been observed in a variety of cancers including melanoma and non-small cell lung cancer. The interaction of PD1/PDL1 is hypothesized to be a possible mechanism for the tumor to escape immune response. A number of checkpoint blockade inhibitors including pembrolizumab and nivolumab have been developed that target the PD1/PDL1 interaction in order to allow T-cells to recognize tumor cells without being deactivated by the tumor.CD274 (CD274 Molecule) is a Protein Coding gene. Diseases associated with CD274 include Testicular Lymphoma and Smoldering Myeloma. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. An important paralog of this gene is PDCD1LG2.Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survivalThe interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

Chromosomal Localization:

9p24.1

Accession Number:

NP_054862.1

UniProt Accession Number:

Q9NZQ7

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

NIIQFVHGEEDLK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1430

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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