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CD27 Molecule Peptide 1

Antibody: CPTC-CD27-1
Background

Catalog Number:

CPTC-CD27-1

Target Antigen:

CD27 Molecule Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

10/31/2023

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (505.5 KB)

CPTC-CD27-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (505.5 KB)

CPTC-CD27-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

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Antibody: CPTC-CD27-2
Background

Catalog Number:

CPTC-CD27-2

Target Antigen:

CD27 Molecule Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

10/31/2023

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (548.4 KB)

CPTC-CD27-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (548.4 KB)

CPTC-CD27-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CD27-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate

CPTC-CD27-2 Western Blot (Over-Expressed Lysate)

Result: Negative

Western blot using CPTC-CD27-2 as primary antibody against transient overexpression lysate of CD27 molecule (CD27). The expected molecular weight is 29.2 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-CD27-2 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-CD27-2 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 29.1 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-CD27-2 Simple Western (Lysates)

Result: Negative

Automated WB (Simple Western) using CPTC-CD27-2 as primary antibody against the whole lysate of PBMC, HeLa, Jurkat, A549, MCF7 and H226. Expected MW is 29 Kda. The antibody does not recognize the target in tested lysates.


Characterization SOP Files

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Antibody: CPTC-CD27-3
Background

Catalog Number:

CPTC-CD27-3

Target Antigen:

CD27 Molecule Peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

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External Links
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image The affinity and binding kinetics of CPTC-CD27-3 and human CD27 recombinant protein were measured using surface plasmon resonance. Buffer exchanged CD27 recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-CD27-3 antibody at, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM and 0.25 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a bivalent fitting model. Click image to enlarge

CPTC-CD27-3 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Positive

The affinity and binding kinetics of CPTC-CD27-3 and human CD27 recombinant protein were measured using surface plasmon resonance. Buffer exchanged CD27 recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-CD27-3 antibody at, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM and 0.25 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a bivalent fitting model.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org) (469.1 KB)

CPTC-CD27-3 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue

CPTC-CD27-3 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org) (469.1 KB)

CPTC-CD27-3 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CD27-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-CD27-3 as primary antibody against human CD27 molecule (CD27) recombinant protein (lane 2). Expected molecular weight - 26.9 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD27-3 Western Blot Recombinant Protein

Result: Presumed Positive (with additional bands)

Western blot using CPTC-CD27-3 as primary antibody against human CD27 molecule (CD27) recombinant protein (lane 2). Expected molecular weight - 26.9 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD27-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 29 kDa. Molecular weight standards are also included (lane 1). PBMC is positive. All other cell lines are negative. Click image to enlarge

CPTC-CD27-3 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-CD27-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 29 kDa. Molecular weight standards are also included (lane 1). PBMC is positive. All other cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD27-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 29.1 kDa. Molecular weight standards are also included (lane 1). MCF7 and NCI-H226 cell lines are positive. All other cell lines are negative. Click image to enlarge

CPTC-CD27-3 Western Blot Cell Lysate

Result: Positive

Western blot using CPTC-CD27-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 29.1 kDa. Molecular weight standards are also included (lane 1). MCF7 and NCI-H226 cell lines are positive. All other cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD27-3 as primary antibody against human lung (2), spleen (3), endometrium (4) , breast (5), and ovary (6) tissue lysates. The expected molecular weight is 29.1 kDa. Vinculin was used as a loading control. Click image to enlarge

CPTC-CD27-3 Western Blot (Tissue Lysates)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-CD27-3 as primary antibody against human lung (2), spleen (3), endometrium (4) , breast (5), and ovary (6) tissue lysates. The expected molecular weight is 29.1 kDa. Vinculin was used as a loading control.


Characterization SOP Files

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Antigen: CD27 Molecule Peptide 1
Background

NCI Identification Number:

00467

Antigen Name:

CD27 Molecule Peptide 1

CPTC Name:

CPTC-CD27 Peptide 1

Aliases:

CD27 Molecule; Tumor Necrosis Factor Receptor Superfamily, Member 7; T-Cell Activation Antigen CD27; CD27L Receptor; CD27 Antigen; TNFRSF7; S152; Tp55; T14; Tumor Necrosis Factor Receptor Superfamily Member 7; T Cell Activation Antigen S152; S152. LPFS2; CD27

Function:

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.CD27 (CD27 Molecule) is a Protein Coding gene. Diseases associated with CD27 include Lymphoproliferative Syndrome 2 and Combined T Cell And B Cell Immunodeficiency. Among its related pathways are TNF Superfamily - Human Ligand-Receptor Interactions and their Associated Functions and Akt Signaling. Gene Ontology (GO) annotations related to this gene include transmembrane signaling receptor activity and cysteine-type endopeptidase inhibitor activity involved in apoptotic process. An important paralog of this gene is TNFRSF9.Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Chromosomal Localization:

12p13.31

Accession Number:

NP_001233.2

UniProt Accession Number:

P26842

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

EEEGSTIPIQEDYR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1540

Last Updated:

12/16/2020

Links

Characterization Data

SOPs

No SOPs available.

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