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CD14 Molecule Peptide 1

Antibody: CPTC-CD14-1
Background

Catalog Number:

CPTC-CD14-1

RRID:

AB_2889954

Target Antigen:

CD14 Molecule Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (346.3 KB)

CPTC-CD14-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image "Immuno-MRM chromatogram of CPTC-CD14-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5944) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-CD14-1 iMRM

Result: Positive

"Immuno-MRM chromatogram of CPTC-CD14-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5944)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence

CPTC-CD14-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-CD14-1 Ab shows no localization of CD14 protein.


Characterization SOP Files

  NCI 60 Protein Array

CTPC-CD14-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-CD14-1 Western Blot (recombinant protein)

Result: Negative

Western blot using CPTC-CD14-1 as primary antibody against human CD14 molecule (CD14), transcript variant 1 recombinant protein (lane 2). Expected molecular weight - 35.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-CD14-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-CD14-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 40.1. Buffy coat and all cell lines are negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD14-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 40.1. All cell lines are presumed positive. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD14-1 Western Blot (Cell Lysate)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-CD14-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 40.1. All cell lines are presumed positive. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: CD14 Molecule Peptide 1
Background

NCI Identification Number:

00350

Antigen Name:

CD14 Molecule Peptide 1

CPTC Name:

CPTC-CD14 Peptide 1

Aliases:

CD14 Molecule; Myeloid Cell-Specific Leucine-Rich Glycoprotein; Monocyte Differentiation Antigen CD14; CD14 Antigen; CD14

Function:

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. CD14 (CD14 Molecule) is a Protein Coding gene. Diseases associated with CD14 include Mycobacterium Chelonae and Mycobacterium Kansasii. Among its related pathways are Antigen processing-Cross presentation and Transcriptional misregulation in cancer. Gene Ontology (GO) annotations related to this gene include lipopolysaccharide binding and lipoteichoic acid binding.Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-)

Chromosomal Localization:

5q31.3

Accession Number:

NP_000582.1

UniProt Accession Number:

P08571

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

FPAIQNLALR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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