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Caspase 3 Peptide 1

Antibody: CPTC-CASP3-1
Background

Catalog Number:

CPTC-CASP3-1

Target Antigen:

Caspase 3 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (347.5 KB)

CPTC-CASP3-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue

CTPC-CASP3-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image "Immuno-MRM chromatogram of CPTC-CASP3-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-597) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)" Click image to enlarge

CPTC-CASP3-1 iMRM

Result: Positive

"Immuno-MRM chromatogram of CPTC-CASP3-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-597)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)"


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-SERPINA6-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5907) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysates Click image to enlarge

CPTC-CASP3-1 iMRM (Cell Lysates)

Result: Positive

Immuno-MRM chromatogram of CPTC-SERPINA6-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5907)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysates


Characterization SOP Files

  NCI 60 Protein Array

CTPC-CASP3-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate

CPTC-CASP3-1 Western Blot (Over-Expressed Lysate)

Result: Negative

Western blot using CPTC-CASP3-1 as primary antibody against caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha, transient overexpression lysate. The expected molecular weight is 31.6 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CASP3-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line. Click image to enlarge

CPTC-CASP3-1 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-CASP3-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-CASP3-1 Western Blot (Cell Lysates)

Result: Negative

Western blot using CPTC-CASP3-1 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 31.6 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

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Antigen: Caspase 3 Peptide 1
Background

NCI Identification Number:

00308

Antigen Name:

Caspase 3 Peptide 1

CPTC Name:

CPTC-CASP3 Peptide 1

Aliases:

Caspase 3; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; Apopain; CASP-3; CPP-32; CPP32; SCA-1; PARP Cleavage Protease; Procaspase3; EC 3.4.22; CPP32B

Function:

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.CASP3 (Caspase 3) is a Protein Coding gene. Diseases associated with CASP3 include Helicobacter Pylori Infection and Fixed Drug Eruption. Among its related pathways are Human cytomegalovirus infection and Apoptosis Modulation and Signaling. Gene Ontology (GO) annotations related to this gene include peptidase activity and cysteine-type peptidase activity. An important paralog of this gene is CASP7.Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Caspases (cysteinyl aspartate proteases) are involved in the signaling pathways of apoptosis, necrosis and inflammation. These enzymes can be divided into initiators and effectors. The initiator isoforms are activated by, and interact with, upstream adaptor molecules.

Chromosomal Localization:

4q35.1

Accession Number:

na

UniProt Accession Number:

P42574

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CIIHGSES(ph)MDSGISLDNSYK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2200

Last Updated:

10/23/2019

Links

Characterization Data

SOPs

No SOPs available.

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