Background
Catalog Number:
CPTC-CADM1-1
Target Antigen:
Cell Adhesion Molecule 1 Peptide 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
02/02/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-CADM1-1
Characterization Data [Compare Characterization Data]
IHC Tissue
CPTC-CADM1-1 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
NCI 60 Protein Array
CPTC-CADM1-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CADM1-1 Western Blot Recombinant Protein
Result: Presumed Positive (with additional bands)
Western blot using CPTC-CADM1-1 as primary antibody against human cell adhesion molecule 1 (CADM1), transcript variant 1, recombinant protein (lane 2). Expected molecular weight - 48.3 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CADM1-1 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-CADM1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 48 kDa and 37 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CADM1-1 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-CADM1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 48.5 kDa, 36.9 kDa, 51.5 kDa, 49.6 kDa, and 45.6 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CADM1-1 Western Blot (Tissue Lysate)
Result: Negative
Western blot using CPTC-CADM1-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 48.5 kDa, 36.9 kDa, 51.5 kDa, 49.6 kDa, and 45.6 kDa. Vinculin was used as a loading control.
Characterization SOP Files
Background
NCI Identification Number:
00465
Antigen Name:
Cell Adhesion Molecule 1 Peptide 1
CPTC Name:
CPTC-CADM1 Peptide 1
Aliases:
Cell Adhesion Molecule 1; Tumor Suppressor In Lung Cancer 1; IGSF4A; SYNCAM; NECL2; Spermatogenic Immunoglobulin Superfamily; Immunoglobulin Superfamily Member 4; Synaptic Cell Adhesion Molecule; Nectin-Like Protein 2; Nectin-Like 2; Necl-2; TSLC-1; IGSF4; RA175; TSLC1; ST17; BL2; Immunoglobulin Superfamily, Member 4D Variant 1; Immunoglobulin Superfamily, Member 4D Variant 2; Immunoglobulin Superfamily, Member 4; TSLC1/Nectin-Like 2/IGSF4; Truncated CADM1 Protein; STSLC-1; SynCAM1; SYNCAM1; SgIGSF; NECL-2; SgIgSF; SynCAM; CADM1; IgSF4
Function:
CADM1 (Cell Adhesion Molecule 1) is a Protein Coding gene. Diseases associated with CADM1 include Retroperitoneal Fibrosis and Acute Myeloid Leukemia With T(9;11)(P22;Q23). Among its related pathways are Cell junction organization and Cell adhesion molecules (CAMs). Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and PDZ domain binding. An important paralog of this gene is CADM2.Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner (PubMed:22438059, PubMed:12050160). Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner (By similarity). Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo (PubMed:15811952). In mast cells, may mediate attachment to and promote communication with nerves (PubMed:15905536). CADM1, together with MITF, is essential for development and survival of mast cells in vivo (PubMed:22438059). By interacting with CRTAM and thus promoting the adhesion between CD8+ T-cells and CD8+ dendritic cells, regulates the retention of activated CD8+ T-cell within the draining lymph node (By similarity). Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells (By similarity). Interaction with CRTAM promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells (By similarity). Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly (By similarity). May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons (By similarity). May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa (By similarity). Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells (PubMed:11279526, PubMed:12234973). May contribute to the less invasive phenotypes of lepidic growth tumor cells.
Chromosomal Localization:
11q23.3
Accession Number:
NP_001287972.1
UniProt Accession Number:
Q9BY67
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
EGDALELTCEAIGKPQPVMVTWVR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2640
Last Updated:
12/16/2020
Links
- EnsEMBL - EnsEMBL CADM1
- Entrez - Entrez CADM1
- Uniprot - Uniprot Q9BY67
Characterization Data
SOPs
No SOPs available.
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