Background
Catalog Number:
CPTC-BTLA-1
Target Antigen:
B And T Lymphocyte Associated Peptide 1
Isotype:
IgG3
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-BTLA-1
Characterization Data [Compare Characterization Data]
NCI 60 Protein Array
CPTC-BTLA-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Over-expressed transient protein in cell lysate
CPTC-BTLA-1 Western Blot (over-expressed lysate)
Result: Negative
Western blot using CPTC-BTLA-1 as primary antibody against the over-expressed lysate of BTLA. The antibody was not able to detect the target protein.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-BTLA-1 Western Blot (cell lysates)
Result: Negative
Western blot using CPTC-BTLA-1 as primary antibody against the whole lysates of SNB-19, A498, BT-549, TK-10, HL60, HS 578T. The antibody was not able to detect the target protein in any of the tested lysates.
Characterization SOP Files
Background
Catalog Number:
CPTC-BTLA-2
Target Antigen:
B And T Lymphocyte Associated Peptide 1
Isotype:
IgG3
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-BTLA-2
Characterization Data [Compare Characterization Data]
NCI 60 Protein Array
CPTC-BTLA-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Over-expressed transient protein in cell lysate
CPTC-BTLA-2 Western Blot (over-expressed lysate)
Result: Negative
Western blot using CPTC-BTLA-2 as primary antibody against the over-expressed lysate of BTLA. The antibody was not able to detect the target protein.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-BTLA-2 Western Blot (cell lysates)
Result: Negative
Western blot using CPTC-BTLA-2 as primary antibody against the whole lysates of SNB-19, A498, BT-549, TK-10, HL60, HS 578T. The antibody was not able to detect the target protein in any of the tested lysates.
Characterization SOP Files
Background
Catalog Number:
CPTC-BTLA-3
Target Antigen:
B And T Lymphocyte Associated Peptide 1
Isotype:
IgG3
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length
Purchase
- DSHB CPTC-BTLA-3
Characterization Data [Compare Characterization Data]
IHC Tissue
CPTC-BTLA-3 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
NCI 60 Protein Array
CPTC-BTLA-3 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-BTLA-3 Western Blot Recombinant Protein
Result: Negative
Western blot using CPTC-BTLA-3 as primary antibody against the over-expressed lysate of BTLA. The antibody was not able to detect the target protein.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-BTLA-3 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-BTLA-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 33 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-BTLA-3 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-BTLA-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 32.8 kDa and 27.3 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-BTLA-3 Western Blot (Tissue Lysates)
Result: Negative
Western blot using CPTC-BTLA-3 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 32.8 kDa and 27.3 kDa. Vinculin was used as a loading control.
Characterization SOP Files
Background
NCI Identification Number:
00463
Antigen Name:
B And T Lymphocyte Associated Peptide 1
CPTC Name:
CPTC-BTLA Peptide 1
Aliases:
B And T Lymphocyte Associated; B- And T-Lymphocyte-Associated Protein; B- And T-Lymphocyte Attenuator; BTLA1; CD272; CD272 Antigen; BTLA
Function:
This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis.BTLA (B And T Lymphocyte Associated) is a Protein Coding gene. Diseases associated with BTLA include Cone-Rod Dystrophy 1 and Rheumatoid Arthritis. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation.Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells
Chromosomal Localization:
3q13.2
Accession Number:
NP_861445.4
UniProt Accession Number:
Q7Z6A9
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
EAPTEYASICVR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1320
Last Updated:
12/16/2020
Links
- EnsEMBL - EnsEMBL BTLA
- Entrez - Entrez BTLA
- Uniprot - Uniprot Q7Z6A9
Characterization Data
SOPs
No SOPs available.
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