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B And T Lymphocyte Associated Peptide 1

Antibody: CPTC-BTLA-1
Background

Catalog Number:

CPTC-BTLA-1

Target Antigen:

B And T Lymphocyte Associated Peptide 1

Isotype:

IgG3

Species:

Mouse Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide

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Characterization Data [Compare Characterization Data]
  NCI 60 Protein Array

CPTC-BTLA-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

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Antibody: CPTC-BTLA-2
Background

Catalog Number:

CPTC-BTLA-2

Target Antigen:

B And T Lymphocyte Associated Peptide 1

Isotype:

IgG3

Species:

Mouse Monoclonal Antibody

Last Updated:

03/01/2024

Antigen Recognition(s):

Peptide

Purchase
Characterization Data [Compare Characterization Data]
  NCI 60 Protein Array

CPTC-BTLA-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

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Antibody: CPTC-BTLA-3
Background

Catalog Number:

CPTC-BTLA-3

Target Antigen:

B And T Lymphocyte Associated Peptide 1

Isotype:

IgG3

Species:

Mouse Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC Tissue

CPTC-BTLA-3 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-BTLA-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-BTLA-3 as primary antibody against human B and T lymphocyte associated (BTLA), transcript variant 1, recombinant protein (lane 2). Expected molecular weight - 32.6 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BTLA-3 Western Blot Recombinant Protein

Result: Presumed Positive (with additional bands)

Western blot using CPTC-BTLA-3 as primary antibody against human B and T lymphocyte associated (BTLA), transcript variant 1, recombinant protein (lane 2). Expected molecular weight - 32.6 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-BTLA-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 33 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BTLA-3 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-BTLA-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 33 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-BTLA-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 32.8 kDa and 27.3 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BTLA-3 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-BTLA-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 32.8 kDa and 27.3 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-BTLA-3 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 32.8 kDa and 27.3 kDa. Vinculin was used as a loading control. Click image to enlarge

CPTC-BTLA-3 Western Blot (Tissue Lysates)

Result: Negative

Western blot using CPTC-BTLA-3 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 32.8 kDa and 27.3 kDa. Vinculin was used as a loading control.


Characterization SOP Files

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Antigen: B And T Lymphocyte Associated Peptide 1
Background

NCI Identification Number:

00463

Antigen Name:

B And T Lymphocyte Associated Peptide 1

CPTC Name:

CPTC-BTLA Peptide 1

Aliases:

B And T Lymphocyte Associated; B- And T-Lymphocyte-Associated Protein; B- And T-Lymphocyte Attenuator; BTLA1; CD272; CD272 Antigen; BTLA

Function:

This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis.BTLA (B And T Lymphocyte Associated) is a Protein Coding gene. Diseases associated with BTLA include Cone-Rod Dystrophy 1 and Rheumatoid Arthritis. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation.Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells

Chromosomal Localization:

3q13.2

Accession Number:

NP_861445.4

UniProt Accession Number:

Q7Z6A9

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

EAPTEYASICVR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

12/16/2020

Links

Characterization Data

SOPs

No SOPs available.

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