Background
Catalog Number:
CPTC-BRAF-3
RRID:
AB_2722026
Target Antigen:
B-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 3
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
11/21/2023
Antigen Recognition(s):
Peptide, Recombinant Full-length
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-BRAF-3
Publications
Characterization Data [Compare Characterization Data]
Affinity Measurement by SPR
CPTC-BRAF-3 Affinity and Kinetics (Surface Plasmon Resonance)
Result: Negative
Affinity and binding kinetics of CPTC-BRAF-3 and full length BRAF recombinant protein were measured using surface plasmon resonance. CPTC-BRAF-3 mouse antibody was captured on a series S protein G biosensor chip. BRAF recombinant protein at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM, was used as analyte.
Characterization SOP Files
Immuno-MRM
CPTC-BRAF-3 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-BRAF-3 antibody with CPTC-BRAF peptide 3 (NCI ID#179) as target
NCI 60 Protein Array
CPTC-BRAF-3 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Over-expressed transient protein in cell lysate
CPTC-BRAF-3 Western Blot
Result: Positive
Western Blot using CPTC-BRAF-3 as primary Ab against recombinant BRAF (lane 2). Also included are molecular wt. standards (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-BRAF-3 Simple Western (cell lysates)
Result: Negative
Simple Western assay using CPTC-BRAF-3 as primary antibody against the whole cell lysates of the cancer cells RPMI-8226, SNB-75, HL-60, NCI/ADR-RES, CCRF-CEM and SR. The target protein BRAF has not been detected in any cell lines. All the cell lines were probed with anti-CytC antibody, and they all show expression of the housekeeping protein at different level.
Characterization SOP Files
Background
NCI Identification Number:
00179
Antigen Name:
B-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 3
CPTC Name:
CPTC-BRAF Peptide 3
Aliases:
B-Raf Proto-Oncogene, Serine/Threonine Kinase; V-Raf Murine Sarcoma Viral Oncogene Homolog B1; V-Raf Murine Sarcoma Viral Oncogene Homolog B; Proto-Oncogene B-Raf; BRAF1; RAFB1; B-Raf Proto-Oncogene Serine/Threonine-Protein Kinase (P94); Murine Sarcoma Viral (V-Raf) Oncogene Homolog B1; Serine/Threonine-Protein Kinase B-Raf; 94 KDa B-Raf Protein; EC 2.7.11.1; B-RAF1; B-Raf; NS7; P94
Function:
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
BRAF (B-Raf Proto-Oncogene, Serine/Threonine Kinase) is a Protein Coding gene. Diseases associated with BRAF include Cardiofaciocutaneous Syndrome and Lung Cancer. Among its related pathways are Development Slit-Robo signaling and MAPK Signaling: Oxidative Stress Pathway. GO annotations related to this gene include calcium ion binding and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is RAF1.
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.
Chromosomal Localization:
7q34
Accession Number:
NP_004324.2
UniProt Accession Number:
P15056
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
AGFQTEDFSLYACASPK
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1870
Last Updated:
12/22/2016
Links
- EnsEMBL - EnsEMBL BRAF
- Entrez - Entrez BRAF
- Uniprot - Uniprot P15056
Characterization Data
SOPs
No SOPs available.
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