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BCL2 Associated Agonist Of Cell Death Peptide 1

Antibody: CPTC-BAD-1
Background

Catalog Number:

CPTC-BAD-1

Target Antigen:

BCL2 Associated Agonist Of Cell Death Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Endogenous

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Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-BAD-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6213) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-BAD-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-BAD-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6213)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array

CPTC-BAD-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-BAD-1 Western Blot (Recombinant Protein)

Result: Negative

Western blot using CPTC-BAD-1 as primary antibody against recombinant protein of human BCL2-associated agonist of cell death (BAD), transcript variant 1 (lane 2). Expected molecular weight - 18.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-BAD-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 18.4 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BAD-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-BAD-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 18.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-BAD-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 18.4 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BAD-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-BAD-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 18.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: BCL2 Associated Agonist Of Cell Death Peptide 1
Background

NCI Identification Number:

00494

Antigen Name:

BCL2 Associated Agonist Of Cell Death Peptide 1

CPTC Name:

CPTC-BAD Peptide 1

Aliases:

BCL2 Associated Agonist Of Cell Death; BCL2L8; Bcl-XL/Bcl-2-Associated Death Promoter; Bcl2-Associated Agonist Of Cell Death; Bcl2 Antagonist Of Cell Death; Bcl-2-Binding Component 6; Bcl-2-Like Protein 8; Bcl2-L-8; BBC2; BCL2-Antagonist Of Cell Death Protein; BCL-X/BCL-2 Binding Protein; BCL2-Binding Component 6; BCL2-Binding Protein; BBC6; BAD

Function:

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.BAD (BCL2 Associated Agonist Of Cell Death) is a Protein Coding gene. Diseases associated with BAD include B-Cell Lymphoma and Myeloid Leukemia. Among its related pathways are Brain-Derived Neurotrophic Factor (BDNF) signaling pathway and IL-9 Signaling Pathways. Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and lipid binding.Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Chromosomal Localization:

11q13.1

Accession Number:

NP_004313.1

UniProt Accession Number:

Q92934

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GLGPSPAGDGPSGSGK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1760

Last Updated:

07/15/2021

Links

Characterization Data

SOPs

No SOPs available.

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