Background
Catalog Number:
CPTC-B2M-1
RRID:
AB_2890076
Target Antigen:
Beta-2-Microglobulin Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
07/03/2024
Antigen Recognition(s):
Peptide, Endogenous
External Links
- CPTAC Assay Portal CPTC-B2M-1
Purchase
- DSHB CPTC-B2M-1
Characterization Data [Compare Characterization Data]
Immuno-MRM
CPTC-B2M-1 iMRM (Frozen Tissue)
Result: Positive
Immuno-MRM chromatogram of CPTC-B2M-1antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6233)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue
Characterization SOP Files
NCI 60 Protein Array
CPTC-B2M-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-B2M-1 Western Blot (Recombinant Protein)
Result: Negative
Western blot using CPTC-B2M-1 as primary antibody against human beta-2-microglobulin (B2M) recombinant protein (lane 2). Expected molecular weight - 11.7 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-B2M-1 Western Blot (Cell Lysate)
Result: Negative
Western blot using CPTC-B2M-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 13.7 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-B2M-1 Automated Western Blot (Cell Lysate)
Result: Negative
Automated western blot using CPTC-B2M-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 13.7 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00346
Antigen Name:
Beta-2-Microglobulin Peptide 1
CPTC Name:
CPTC-B2M Peptide 1
Aliases:
Beta-2-Microglobulin; Beta Chain Of MHC Class I Molecules; Beta-2-Microglobin; IMD43; B2M
Function:
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.B2M (Beta-2-Microglobulin) is a Protein Coding gene. Diseases associated with B2M include Immunodeficiency 43 and Amyloidosis, Familial Visceral. Among its related pathways are Antigen processing-Cross presentation and Interferon gamma signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding. An important paralog of this gene is HLA-DPA1.Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
Chromosomal Localization:
15q21.1
Accession Number:
NP_004039.1
UniProt Accession Number:
P61769
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
VNHVTLSQPK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1100
Last Updated:
09/02/2020
Links
- EnsEMBL - EnsEMBL B2M
- Entrez - Entrez B2M
- Uniprot - Uniprot P61769
Characterization Data
SOPs
No SOPs available.
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