ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1 Peptide 1


Background

Catalog Number:

CPTC-ASAP1-1

RRID:

AB_2877107

Target Antigen:

ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1 Peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

05/27/2021

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]
  Immunofluorescence

CPTC-ASAP1-1

Result: Negative

Immunofluorescence staining of human cell line OVCAR-8 with CPTC-ASAP1-1 Ab shows no localization of ASAP1 protein.


  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-ASAP1-1 as primary antibody against PZA antigen (red) and ZA antigen (blue). Click image to enlarge

CPTC-ASAP1-1 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-ASAP1-1 as primary antibody against PZA antigen (red) and ZA antigen (blue).


Characterization SOP Files

  Western Blot - Cell Lysate
Click to enlarge image Western blot using CPTC-ASAP1-1 as primary antibody agaisnt cell lysates of OVCAR-3, OVCAR-4, OVCAR-8 and SK-OV-3. Molecular weight standards are also included. ASAP1 expected M.W. is ~125 KDa. Click image to enlarge

CPTC-ASAP1-1 Cell Lysate Western Blot

Result: Positive

Western blot using CPTC-ASAP1-1 as primary antibody agaisnt cell lysates of OVCAR-3, OVCAR-4, OVCAR-8 and SK-OV-3. Molecular weight standards are also included. ASAP1 expected M.W. is ~125 KDa.


Characterization SOP Files

  Western Blot - Cell Lysate

CPTC-ASAP1-1 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-ASAP1-1 as a primary antibody against cell lysates. ASAP1 protein was not detected and therefore no expression graphs were generated.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-ASAP1-1 as primary antibody against PZA protein domain (Lane 1, Expected M.W. 45 kDa) and ZA protein domain (Lane 2, Expected M.W. 32 kDa). Molecular weight standards are included for each protein. Click image to enlarge

CPTC-ASAP1-1 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-ASAP1-1 as primary antibody against PZA protein domain (Lane 1, Expected M.W. 45 kDa) and ZA protein domain (Lane 2, Expected M.W. 32 kDa). Molecular weight standards are included for each protein.


Characterization SOP Files

Background

Catalog Number:

CPTC-ASAP1-2

RRID:

AB_2877108

Target Antigen:

ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1 Peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

05/27/2021

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]
  Immunofluorescence

CPTC-ASAP1-2

Result: Negative

Immunofluorescence staining of human cell line OVCAR-8 with CPTC-ASAP1-2 Ab shows no localization of ASAP1 protein.


  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-ASAP1-2 as primary antibody against PZA antigen (red) and ZA antigen (blue). Click image to enlarge

CPTC-ASAP1-2 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-ASAP1-2 as primary antibody against PZA antigen (red) and ZA antigen (blue).


Characterization SOP Files

  Western Blot - Cell Lysate
Click to enlarge image Western blot using CPTC-ASAP1-2 as primary antibody against cell lysates of OVCAR-3, OVCAR-4, OVCAR-8 and SK-OV-3. Weak band for OVCAR-8. ASAP1 expected MW is 125 KDa. Click image to enlarge

CPTC-ASAP1-2 Cell Lysate Western Blot

Result: Positive

Western blot using CPTC-ASAP1-2 as primary antibody against cell lysates of OVCAR-3, OVCAR-4, OVCAR-8 and SK-OV-3. Weak band for OVCAR-8. ASAP1 expected MW is 125 KDa.


Characterization SOP Files

  Western Blot - Cell Lysate

CPTC-ASAP1-2 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-ASAP1-2 as a primary antibody against cell lysates. ASAP1 protein was not detected and therefore no expression graphs were generated.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-ASAP1-2 as primary antibody against PZA protein domain (Lane 1, Expected M.W. 45 kDa) and ZA protein domain (Lane 2, Expected M.W. 32 kDa). Molecular weight standards are included for each protein. Click image to enlarge

CPTC-ASAP1-2 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-ASAP1-2 as primary antibody against PZA protein domain (Lane 1, Expected M.W. 45 kDa) and ZA protein domain (Lane 2, Expected M.W. 32 kDa). Molecular weight standards are included for each protein.


Characterization SOP Files

Background

Catalog Number:

CPTC-ASAP1-3

RRID:

AB_2877109

Target Antigen:

ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1 Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

05/27/2021

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]
  Immunofluorescence

CPTC-ASAP1-3

Result: Negative

Immunofluorescence staining of human cell line OVCAR-8 with CPTC-ASAP1-3 Ab shows no localization of ASAP1 protein.


  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-ASAP1-3 as primary antibody against PZA antigen (red) and ZA antigen (blue). Click image to enlarge

CPTC-ASAP1-3 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-ASAP1-3 as primary antibody against PZA antigen (red) and ZA antigen (blue).


Characterization SOP Files

  Western Blot - Cell Lysate
Click to enlarge image Western blot using CPTC-ASAP1-1 as primary antibody against cell lysates of OVCAR-3, OVCAR-4, OVCAR-8 and SK-OV-3. Molecular weight standards are also included. ASAP1 expected MW is 125 KDa. Click image to enlarge

CPTC-ASAP1-3 Cell Lysate Western Blot

Result: Positive

Western blot using CPTC-ASAP1-1 as primary antibody against cell lysates of OVCAR-3, OVCAR-4, OVCAR-8 and SK-OV-3. Molecular weight standards are also included. ASAP1 expected MW is 125 KDa.


Characterization SOP Files

  Western Blot - Cell Lysate

CPTC-ASAP1-3 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-ASAP1-3 as a primary antibody against cell lysates. ASAP1 protein was not detected and therefore no expression graphs were generated.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-ASAP1-3 as primary antibody against PZA protein domain (Lane 1, Expected M.W. 45 kDa) and ZA protein domain (Lane 2, Expected M.W. 32 kDa). Molecular weight standards are included for each protein. Click image to enlarge

CPTC-ASAP1-3 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-ASAP1-3 as primary antibody against PZA protein domain (Lane 1, Expected M.W. 45 kDa) and ZA protein domain (Lane 2, Expected M.W. 32 kDa). Molecular weight standards are included for each protein.


Characterization SOP Files

Background

NCI Identification Number:

00343

Antigen Name:

ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1 Peptide 1

CPTC Name:

CPTC-ASAP1 Peptide 1

Keywords:

ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1; 130 KDa Phosphatidylinositol 4,5-Bisphosphate-Dependent ARF1 GTPase-Activating Protein; Arf-GAP With SH3 Domain, ANK Repeat And PH Domain-Containing Protein 1; ADP-Ribosylation Factor-Directed GTPase-Activating Protein 1; Development And Differentiation-Enhancing Factor 1; ARF GTPase-Activating Protein 1; PIP2-Dependent ARF1 GAP; Centaurin, Beta 4; KIAA1249; CENTB4; DDEF1; ZG14P; DEF-1; PAP; 130 KDa Phosphatidylinositol 4,5-Biphosphate-Dependent ARF1 GTPase-Activating Protein; Development And Differentiation Enhancing Factor 1; Differentiation-Enhancing Factor 1; AMAP1; ASAP1; PAG2

Function:

This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. ASAP1 (ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 1) is a Protein Coding gene. Diseases associated with ASAP1 include Laryngeal Benign Neoplasm and Melanoma, Uveal. Among its related pathways are Organelle biogenesis and maintenance and EGF/EGFR Signaling Pathway. Gene Ontology (GO) annotations related to this gene include GTPase activator activity and phosphatidylinositol-3,4,5-trisphosphate binding. An important paralog of this gene is ASAP2.Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.

Chromosomal Localization:

8q24.21-q24.22

NCBI Accession Number:

NP_060952.2

UniProt Accession Number:

Q9ULH1

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CANLPSPSPKPTPSSD

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1760

Last Updated:

09/01/2020

Links

Characterization Data

SOPs

No SOPs available.

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