Arginase 2 Peptide 1


Background

Catalog Number:

CPTC-ARG2-1

RRID:

AB_2889952

Target Antigen:

Arginase 2 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

08/26/2022

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (392.3 KB)

CPTC-ARG2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  IHC Tissue

CPTC-ARG2-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  Immunofluorescence

CPTC-ARG2-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-ARG2-1 Ab shows no localization of ARG2 protein.


  NCI 60 Protein Array

CTPC-ARG2-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide

CPTC-ARG2-1 Western Blot (recombinant protein)

Result: Negative

Western blot using CPTC-ARG2-1 as primary antibody against human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein recombinant protein (lane 2). Expected molecular weight - 36 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-ARG2-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates.  Expected molecular weight - 38.6. Buffy coat is positive. All cell lines are negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ARG2-1 Automated Western Blot (Cell Lysate)

Result: Positive

Automated western blot using CPTC-ARG2-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 38.6. Buffy coat is positive. All cell lines are negative. Molecular weight standards are also included (lane 1).


  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-ARG2-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates.  Expected molecular weight - 38.6.  Molecular weight standards are also included (lane 1). All cell lines are positive. Click image to enlarge

CPTC-ARG2-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-ARG2-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 38.6. Molecular weight standards are also included (lane 1). All cell lines are positive.


Characterization SOP Files

Background

NCI Identification Number:

00345

Antigen Name:

Arginase 2 Peptide 1

CPTC Name:

CPTC-ARG2 Peptide 1

Aliases:

Arginase 2; Arginase-2, Mitochondrial; Kidney-Type Arginase; Non-Hepatic Arginase; Arginase, Type II; Type II Arginase; Arginase II; EC 3.5.3.1; L-Arginine Amidinohydrolase; L-Arginine Ureahydrolase; Nonhepatic Arginase; Kidney Arginase; EC 3.5.3; ARG2

Function:

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described.ARG2 (Arginase 2) is a Protein Coding gene. Diseases associated with ARG2 include Argininemia and Impotence. Among its related pathways are Arginine biosynthesis and Viral mRNA Translation. Gene Ontology (GO) annotations related to this gene include arginase activity. An important paralog of this gene is ARG1.May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells (PubMed:27745970). May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed:27214549). May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (By similarity). In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF-alpha production during gestation (PubMed:28614294). Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed:22928666). Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed:25484082). Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (PubMed:23832324). Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal (PubMed:12859189).

Chromosomal Localization:

14q24.1

Accession Number:

NP_001163.1

UniProt Accession Number:

P78540

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

TFDLLIGK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

880

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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