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Arginase 1 Peptide 1

Antibody: CPTC-ARG1-1
Background

Catalog Number:

CPTC-ARG1-1

Target Antigen:

Arginase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

05/23/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on normal spleen tissue core using CPTC-ARG1-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (bone marrow, spleen, placenta, testis, and endometrium) and cancer tissues (breast, ovarian, and lung). Click image to enlarge

CPTC-ARG1-1 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on normal spleen tissue core using CPTC-ARG1-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (bone marrow, spleen, placenta, testis, and endometrium) and cancer tissues (breast, ovarian, and lung).


Characterization SOP Files

  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (331.1 KB)

CPTC-ARG1-1 evaluation by the Human Protein Atlas

Result: Positive

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (205.0 KB)

CPTC-ARG1-1 evaluation by the Human Protein Atlas

Result: Positive

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon showing nuclear staining using Antibody CPTC-ARG1-1. Titer: 1:200 Click image to enlarge

CPTC-ARG1-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon showing nuclear staining using Antibody CPTC-ARG1-1. Titer: 1:200


Characterization SOP Files

  NCI 60 Protein Array

CPTC-ARG1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-ARG1-1 as primary antibody against human Arginase-1 (1-322, His-tag) recombinant protein (lane 2). Expected molecular weight - 35.8 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ARG1-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-ARG1-1 as primary antibody against human Arginase-1 (1-322, His-tag) recombinant protein (lane 2). Expected molecular weight - 35.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-ARG1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 35 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ARG1-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-ARG1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 35 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-ARG1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 34.7 kDa, 35.7 kDa, and 25.4 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ARG1-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-ARG1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 34.7 kDa, 35.7 kDa, and 25.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Arginase 1 Peptide 1
Background

NCI Identification Number:

00431

Antigen Name:

Arginase 1 Peptide 1

CPTC Name:

CPTC-ARG1 Peptide 1

Aliases:

Arginase 1; Liver-Type Arginase; Arginase, Liver; Type I Arginase; Arginase-1; EC 3.5.3.1; ARG1

Function:

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. ARG1 (Arginase 1) is a Protein Coding gene. Diseases associated with ARG1 include Argininemia and Intestinal Schistosomiasis. Among its related pathways are Arginine biosynthesis and IL4-mediated signaling events. Gene Ontology (GO) annotations related to this gene include manganese ion binding and arginase activity. An important paralog of this gene is ARG2.Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Chromosomal Localization:

6q23.2

Accession Number:

NP_001231367.1

UniProt Accession Number:

P05089

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DVDPGEHYILK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

09/24/2020

Links

Characterization Data

SOPs

No SOPs available.

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