A-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 5


Background

Catalog Number:

CPTC-ARAF-4

Target Antigen:

A-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 5

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Peptide, Recombinant Full-length

Thematic Panel(s):

Ras Pathway

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-ARAF-4 antibody with CPTC-ARAF peptide 5 (NCI ID#00149) as target Click image to enlarge

CPTC-ARAF-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-ARAF-4 antibody with CPTC-ARAF peptide 5 (NCI ID#00149) as target


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-ARAF-1 as primary Ab against recombinant ARAF (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-ARAF-4 Western Blot

Result: Positive

Western Blot using CPTC-ARAF-1 as primary Ab against recombinant ARAF (lane 2). Also included are molecular wt. standards (lane 1).


Background

NCI Identification Number:

00149

Antigen Name:

A-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 5

CPTC Name:

CPTC-ARAF Peptide 5

Keywords:

A-Raf Proto-Oncogene, Serine/Threonine Kinase; V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1; Proto-Oncogene A-Raf-1; Proto-Oncogene Pks; EC 2.7.11.1; ARAF1; PKS2; Ras-Binding Protein DA-Raf; Proto-Oncogene A-Raf; Oncogene ARAF1; EC 2.7.11; A-RAF; RAFA1; PKS

Function:

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

ARAF (A-Raf Proto-Oncogene, Serine/Threonine Kinase) is a Protein Coding gene. Diseases associated with ARAF include pallister-killian syndrome and mycobacterium marinum. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Signaling by GPCR. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is RAF1.

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.

Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.

Chromosomal Localization:

Xp11.3

NCBI Accession Number:

NP_001243125.1

Swiss Prot Accession Number:

P10398

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DSGYYWEVPPSEVQLLK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1870

Last Updated:

11/14/2019

Links

Characterization Data

SOPs

No SOPs available.

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