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Apolipoprotein B

Antibody: CPTC-APOB-1
Background

Catalog Number:

CPTC-APOB-1

Target Antigen:

Apolipoprotein B

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Endogenous

External Links
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-APOB-1 and APOB 100 native protein were measured using biolayer interferometry. CPTC-APOB-1 antibody was covalently immobilized on amine-reactive second-generation sensors. APOB 100 native protein, 16 nM, 4 nM and 1 nM was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. Click image to enlarge

CPTC-APOB-1 Affinity and Kinetics (Biolayer Interferometry)

Result: Positive

The affinity and binding kinetics of CPTC-APOB-1 and APOB 100 native protein were measured using biolayer interferometry. CPTC-APOB-1 antibody was covalently immobilized on amine-reactive second-generation sensors. APOB 100 native protein, 16 nM, 4 nM and 1 nM was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-APOB-1 and Apolipoprotein B / Apo B (APOB 100) human protein was measured using surface plasmon resonance. CPTC-APOB-1 was captured onto a Series S Protein G biosensor chip. APOB 100 native protein, 64 nM, 16 nM, 4 nM, and 1 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a 1:1 model. APOB 100 native protein bound non-specifically to the Protein G reference channel (Fc1). The NSB was ~ 90% of the signal observed on the active channel (Fc2). Click image to enlarge

CPTC-APOB-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Presumed Positive

Affinity and binding kinetics of CPTC-APOB-1 and Apolipoprotein B / Apo B (APOB 100) human protein was measured using surface plasmon resonance. CPTC-APOB-1 was captured onto a Series S Protein G biosensor chip. APOB 100 native protein, 64 nM, 16 nM, 4 nM, and 1 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a 1:1 model. APOB 100 native protein bound non-specifically to the Protein G reference channel (Fc1). The NSB was ~ 90% of the signal observed on the active channel (Fc2).


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (547.7 KB)

CPTC-APOB-1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue

CPTC-APOB-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IP
Click to enlarge image IP-WB performed with CPTC-APOB-1 antibody as capture antibody. Eluates were screened in WB using antibody CPTC-APOB-3 as detection antibody. CPTC-APOB-1 was able to pull down the native protein purified from human plasma and the protein in human plasma, however, does not show to be able to pull down the target in HepG2 lysate. APOB MW is 515 KDa. Click image to enlarge

CPTC-APOB-1 IP-WB

Result: Positive

IP-WB performed with CPTC-APOB-1 antibody as capture antibody. Eluates were screened in WB using antibody CPTC-APOB-3 as detection antibody. CPTC-APOB-1 was able to pull down the native protein purified from human plasma and the protein in human plasma, however, does not show to be able to pull down the target in HepG2 lysate. APOB MW is 515 KDa.


Characterization SOP Files

  Immunofluorescence

CPTC-APOB-1 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-APOB-1 as primary antibody against HepG2 cells shows no localization of APOB protein.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (547.7 KB)

CPTC-APOB-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-APOB-1 as primary antibody against human Apolipoprotein B / Apo B (APOB 100) native protein. Click image to enlarge

CPTC-APOB-1 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-APOB-1 as primary antibody against human Apolipoprotein B / Apo B (APOB 100) native protein.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-APOB-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-APOB-1 as primary antibody against human APOB 100 native protein (lane 2). Expected molecular weight - 516 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-APOB-1 Western Blot (Native Protein)

Result: Positive

Western blot using CPTC-APOB-1 as primary antibody against human APOB 100 native protein (lane 2). Expected molecular weight - 516 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-APOB-1 as primary antibody against COLO205 (lane 2), HCC-2998 (lane 3), HCT-116 (lane 4), HCT-15 (lane 5), HT-29 (lane 6), SW-620 (lane 7), CaCO2 (lane 8), Hep G2 (lane 9) whole cell lysates and human (lane 10), mouse (lane 11), bovine (lane 12), and rabbit (lane 13) plasma. Expected molecular weight – 516 kDa. Molecular weight standards are also included (lane 1). Human plasma is positive. Click image to enlarge

CPTC-APOB-1 Western Blot (Cell Lysate and Plasma)

Result: Positive

Western blot using CPTC-APOB-1 as primary antibody against COLO205 (lane 2), HCC-2998 (lane 3), HCT-116 (lane 4), HCT-15 (lane 5), HT-29 (lane 6), SW-620 (lane 7), CaCO2 (lane 8), Hep G2 (lane 9) whole cell lysates and human (lane 10), mouse (lane 11), bovine (lane 12), and rabbit (lane 13) plasma. Expected molecular weight – 516 kDa. Molecular weight standards are also included (lane 1). Human plasma is positive.


Characterization SOP Files

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Antibody: CPTC-APOB-2
Background

Catalog Number:

CPTC-APOB-2

Target Antigen:

Apolipoprotein B

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Endogenous

External Links
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-APOB-2 and APOB 100 native protein were measured using biolayer interferometry. CPTC-APOB-2 antibody was covalently immobilized on amine-reactive second-generation sensors. APOB 100 native protein, 256 nM, 64 nM, 16 nM and 4 nM was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. Click image to enlarge

CPTC-APOB-2 Affinity and Kinetics (Biolayer Interferometry)

Result: Positive

The affinity and binding kinetics of CPTC-APOB-2 and APOB 100 native protein were measured using biolayer interferometry. CPTC-APOB-2 antibody was covalently immobilized on amine-reactive second-generation sensors. APOB 100 native protein, 256 nM, 64 nM, 16 nM and 4 nM was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-APOB-2 and Apolipoprotein B / Apo B (APOB 100) human protein were measured using surface plasmon resonance. CPTC-APOB-2 was captured onto a Series S Protein G biosensor chip. APOB 100 native protein, 64 nM, 16 nM, 4 nM, and 1 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a 1:1 model. Kinetic constant, Kd, is outside the limits that can be measured by the instrument due to non-specific binding of the native APOB 100 protein to the Protein G reference surface. Kinetic constants cannot be uniquely determined due to non-specific binding of the native APOB 100 protein to the Protein G reference surface. Click image to enlarge

CPTC-APOB-2 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Presumed Positive

Affinity and binding kinetics of CPTC-APOB-2 and Apolipoprotein B / Apo B (APOB 100) human protein were measured using surface plasmon resonance. CPTC-APOB-2 was captured onto a Series S Protein G biosensor chip. APOB 100 native protein, 64 nM, 16 nM, 4 nM, and 1 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a 1:1 model. Kinetic constant, Kd, is outside the limits that can be measured by the instrument due to non-specific binding of the native APOB 100 protein to the Protein G reference surface. Kinetic constants cannot be uniquely determined due to non-specific binding of the native APOB 100 protein to the Protein G reference surface.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (546.3 KB)

CPTC-APOB-2 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue

CPTC-APOB-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IP
Click to enlarge image IP-WB performed with CPTC-APOB-2 antibody as capture antibody. Eluates were screened in WB using antibody CPTC-APOB-3 as detection antibody. CPTC-APOB-2 was able to pull down the native protein purified from human plasma, in HepG2 lysate and the protein in human plasma. APOB MW is 515 KDa. Click image to enlarge

CPTC-APOB-2 IP-WB

Result: Positive

IP-WB performed with CPTC-APOB-2 antibody as capture antibody. Eluates were screened in WB using antibody CPTC-APOB-3 as detection antibody. CPTC-APOB-2 was able to pull down the native protein purified from human plasma, in HepG2 lysate and the protein in human plasma. APOB MW is 515 KDa.


Characterization SOP Files

  Immunofluorescence

CPTC-APOB-2 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-APOB-2 as primary antibody against HepG2 cells shows no localization of APOB protein.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (546.3 KB)

CPTC-APOB-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-APOB-2 as primary antibody against human Apolipoprotein B / Apo B (APOB 100) native protein. Click image to enlarge

CPTC-APOB-2 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-APOB-2 as primary antibody against human Apolipoprotein B / Apo B (APOB 100) native protein.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-APOB-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-APOB-2 as primary antibody against human APOB 100 native protein (lane 2). Expected molecular weight - 516 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-APOB-2 Western Blot (Native Protein)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-APOB-2 as primary antibody against human APOB 100 native protein (lane 2). Expected molecular weight - 516 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-APOB-2 as primary antibody against COLO205 (lane 2), HCC-2998 (lane 3), HCT-116 (lane 4), HCT-15 (lane 5), HT-29 (lane 6), SW-620 (lane 7), CaCO2 (lane 8), Hep G2 (lane 9) whole cell lysates and human (lane 10), mouse (lane 11), bovine (lane 12), and rabbit (lane 13) plasma. Expected molecular weight – 516 kDa. Molecular weight standards are also included (lane 1). HepG2 and human plasma are positive. Click image to enlarge

CPTC-APOB-2 Western Blot (Cell Lysate and Plasma)

Result: Positive

Western blot using CPTC-APOB-2 as primary antibody against COLO205 (lane 2), HCC-2998 (lane 3), HCT-116 (lane 4), HCT-15 (lane 5), HT-29 (lane 6), SW-620 (lane 7), CaCO2 (lane 8), Hep G2 (lane 9) whole cell lysates and human (lane 10), mouse (lane 11), bovine (lane 12), and rabbit (lane 13) plasma. Expected molecular weight – 516 kDa. Molecular weight standards are also included (lane 1). HepG2 and human plasma are positive.


Characterization SOP Files

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Antibody: CPTC-APOB-3
Background

Catalog Number:

CPTC-APOB-3

Target Antigen:

Apolipoprotein B

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Endogenous

External Links
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-APOB-3 and APOB 100 native protein were measured using biolayer interferometry. CPTC-APOB-3 antibody was covalently immobilized on amine-reactive second-generation sensors. APOB 100 native protein, 256 nM, 64 nM, 16 nM and 4 nM was used as analyte. Buffer only and biosensors immobilized without antibody were used for reference subtraction. Click image to enlarge

CPTC-APOB-3 Affinity and Kinetics (Biolayer Interferometry)

Result: Positive

The affinity and binding kinetics of CPTC-APOB-3 and APOB 100 native protein were measured using biolayer interferometry. CPTC-APOB-3 antibody was covalently immobilized on amine-reactive second-generation sensors. APOB 100 native protein, 256 nM, 64 nM, 16 nM and 4 nM was used as analyte. Buffer only and biosensors immobilized without antibody were used for reference subtraction.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-APOB-3 and Apolipoprotein B / Apo B (APOB 100) human protein were measured using surface plasmon resonance. CPTC-APOB-3 was captured onto a Series S Protein G biosensor chip. APOB 100 native protein, 64 nM, 16 nM, 4 nM, and 1 nM, was used as analyte. No binding was observed. Click image to enlarge

CPTC-APOB-3 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-APOB-3 and Apolipoprotein B / Apo B (APOB 100) human protein were measured using surface plasmon resonance. CPTC-APOB-3 was captured onto a Series S Protein G biosensor chip. APOB 100 native protein, 64 nM, 16 nM, 4 nM, and 1 nM, was used as analyte. No binding was observed.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (483.1 KB)

CPTC-APOB-3 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue

CPTC-APOB-3 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IP
Click to enlarge image IP-WB performed with CPTC-APOB-3 antibody as capture antibody. Eluates were screened in WB using antibody CPTC-APOB-3 as detection antibody. CPTC-APOB-3 was able to pull down the native protein purified from human plasma, in HepG2 lysate and the protein in human plasma. APOB MW is 515 KDa. Click image to enlarge

CPTC-APOB-2 IP-WB

Result: Positive

IP-WB performed with CPTC-APOB-3 antibody as capture antibody. Eluates were screened in WB using antibody CPTC-APOB-3 as detection antibody. CPTC-APOB-3 was able to pull down the native protein purified from human plasma, in HepG2 lysate and the protein in human plasma. APOB MW is 515 KDa.


Characterization SOP Files

  Immunofluorescence

CPTC-APOB-3 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-APOB-3 as primary antibody against HepG2 cells shows no localization of APOB protein.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (483.1 KB)

CPTC-APOB-3 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-APOB-3 as primary antibody against human Apolipoprotein B / Apo B (APOB 100) native protein. Click image to enlarge

CPTC-APOB-3 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-APOB-3 as primary antibody against human Apolipoprotein B / Apo B (APOB 100) native protein.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-APOB-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-APOB-3 as primary antibody against human APOB 100 native protein (lane 2). Expected molecular weight - 516 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-APOB-3 Western Blot (Native Protein)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-APOB-3 as primary antibody against human APOB 100 native protein (lane 2). Expected molecular weight - 516 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-APOB-3 as primary antibody against COLO205 (lane 2), HCC-2998 (lane 3), HCT-116 (lane 4), HCT-15 (lane 5), HT-29 (lane 6), SW-620 (lane 7), CaCO2 (lane 8), Hep G2 (lane 9) whole cell lysates and human (lane 10), mouse (lane 11), bovine (lane 12), and rabbit (lane 13) plasma. Expected molecular weight – 516 kDa. Molecular weight standards are also included (lane 1). Hep G2 and human plasma are positive. Click image to enlarge

CPTC-APOB-3 Western Blot (Cell Lysate and Plasma)

Result: Positive

Western blot using CPTC-APOB-3 as primary antibody against COLO205 (lane 2), HCC-2998 (lane 3), HCT-116 (lane 4), HCT-15 (lane 5), HT-29 (lane 6), SW-620 (lane 7), CaCO2 (lane 8), Hep G2 (lane 9) whole cell lysates and human (lane 10), mouse (lane 11), bovine (lane 12), and rabbit (lane 13) plasma. Expected molecular weight – 516 kDa. Molecular weight standards are also included (lane 1). Hep G2 and human plasma are positive.


Characterization SOP Files

[top]

Antigen: Apolipoprotein B
Background

NCI Identification Number:

00507

Antigen Name:

Apolipoprotein B

CPTC Name:

CPTC-APOB

Aliases:

Apolipoprotein B; Apolipoprotein B (Including Ag(X) Antigen); Apolipoprotein B-100; Apolipoprotein B48; Apo B-100; ApoB-100; ApoB-48; LDLCQ4; FCHL2; FLDB

Function:

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL), and is the ligand for the LDL receptor. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. APOB (Apolipoprotein B) is a Protein Coding gene. Diseases associated with APOB include Hypobetalipoproteinemia, Familial, 1 and Hypercholesterolemia, Familial, 2. Among its related pathways are Folate Metabolism and Activated TLR4 signalling. Gene Ontology (GO) annotations related to this gene include binding and heparin binding.Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Chromosomal Localization:

2p24.1

Accession Number:

NP_000375.3

UniProt Accession Number:

P04114

DNA Source:

N/A

Immunogen:

Native Protein

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

MDPPRPALLALLALPALLLLLLAGARAEEEMLENVSLVCPKDAT RFKHL
RKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTL
KEV YGFNPEGKALLKKTKNSEEFAAAMSRYELKLAIPEGKQVFLYPEKD
EPTYILNIKRGI ISALLVPPETEEAKQVLFLDTVYGNCSTHFTVKTRKG
NVATEISTERDLGQCDRFKPI RTGISPLALIKGMTRPLSTLISSSQSCQ
YTLDAKRKHVAEAICKEQHLFLPFSYKNKY GMVAQVTQTLKLEDTPKIN
SRFFGEGTKKMGLAFESTKSTSPPKQAEAVLKTLQELKK LTISEQNIQR
ANLFNKLVTELRGLSDEAVTSLLPQLIEVSSPITLQALVQCGQPQCST H
ILQWLKRVHANPLLIDVVTYLVALIPEPSAQQLREIFNMARDQRSRATLY
ALSHAVN NYHKTNPTGTQELLDIANYLMEQIQDDCTGDEDYTYLILRVI
GNMGQTMEQLTPELKS SILKCVQSTKPSLMIQKAAIQALRKMEPKDKDQ
EVLLQTFLDDASPGDKRLAAYLMLM RSPSQADINKIVQILPWEQNEQVK
NFVASHIANILNSEELDIQDLKKLVKEALKESQL PTVMDFRKFSRNYQL
YKSVSLPSLDPASAKIEGNLIFDPNNYLPKESMLKTTLTAFGF ASADLI
EIGLEGKGFEPTLEALFGKQGFFPDSVNKALYWVNGQVPDGVSKVLVDHF
GY TKDDKHEQDMVNGIMLSVEKLIKDLKSKEVPEARAYLRILGEELGFA
SLHDLQLLGKL LLMGARTLQGIPQMIGEVIRKGSKNDFFLHYIFMENAF
ELPTGAGLQLQISSSGVIAP GAKAGVKLEVANMQAELVAKPSVSVEFVT
NMGIIIPDFARSGVQMNTNFFHESGLEAH VALKAGKLKFIIPSPKRPVK
LLSGGNTLHLVSTTKTEVIPPLIENRQSWSVCKQVFPG LNYCTSGAYSN
ASSTDSASYYPLTGDTRLELELRPTGEIEQYSVSATYELQREDRALV DT
LKFVTQAEGAKQTEATMTFKYNRQSMTLSSEVQIPDFDVDLGTILRVNDE
STEGKT SYRLTLDIQNKKITEVALMGHLSCDTKEERKIKGVISIPRLQA
EARSEILAHWSPAKL LLQMDSSATAYGSTVSKRVAWHYDEEKIEFEWNT
GTNVDTKKMTSNFPVDLSDYPKSL HMYANRLLDHRVPQTDMTFRHVGSK
LIVAMSSWLQKASGSLPYTQTLQDHLNSLKEFN LQNMGLPDFHIPENLF
LKSDGRVKYTLNKNSLKIEIPLPFGGKSSRDLKMLETVRTPA LHFKSVG
FHLPSREFQVPTFTIPKLYQLQVPLLGVLDLSTNVYSNLYNWSASYSGGN
T STDHFSLRARYHMKADSVVDLLSYNVQGSGETTYDHKNTFTLSCDGSL
RHKFLDSNIK FSHVEKLGNNPVSKGLLIFDASSSWGPQMSASVHLDSKK
KQHLFVKEVKIDGQFRVSS FYAKGTYGLSCQRDPNTGRLNGESNLRFNS
SYLQGTNQITGRYEDGTLSLTSTSDLQS GIIKNTASLKYENYELTLKSD
TNGKYKNFATSNKMDMTFSKQNALLRSEYQADYESLR FFSLLSGSLNSH
GLELNADILGTDKINSGAHKATLRIGQDGISTSATTNLKCSLLVLE NEL
NAELGLSGASMKLTTNGRFREHNAKFSLDGKAALTELSLGSAYQAMILGV
DSKNI FNFKVSQEGLKLSNDMMGSYAEMKFDHTNSLNIAGLSLDFSSKL
DNIYSSDKFYKQTV NLQLQPYSLVTTLNSDLKYNALDLTNNGKLRLEPL
KLHVAGNLKGAYQNNEIKHIYAI SSAALSASYKADTVAKVQGVEFSHRL
NTDIAGLASAIDMSTNYNSDSLHFSNVFRSVM APFTMTIDAHTNGNGKL
ALWGEHTGQLYSKFLLKAEPLAFTFSHDYKGSTSHHLVSRK SISAALEH
KVSALLTPAEQTGTWKLKTQFNNNEYSQDLDAYNTKDKIGVELTGRTLAD
LTLLDSPIKVPLLLSEPINIIDALEMRDAVEKPQEFTIVAFVKYDKNQD
VHSINLPFF ETLQEYFERNRQTIIVVLENVQRNLKHINIDQFVRKYRAA
LGKLPQQANDYLNSFNWE RQVSHAKEKLTALTKKYRITENDIQIALDDA
KINFNEKLSQLQTYMIQFDQYIKDSYD LHDLKIAIANIIDEIIEKLKSL
DEHYHIRVNLVKTIHDLHLFIENIDFNKSGSSTASW IQNVDTKYQIRIQ
IQEKLQQLKRHIQNIDIQHLAGKLKQHIEAIDVRVLLDQLGTTIS FERI
NDILEHVKHFVINLIGDFEVAEKINAFRAKVHELIERYEVDQQIQVLMDK
LVEL AHQYKLKETIQKLSNVLQQVKIKDYFEKLVGFIDDAVKKLNELSF
KTFIEDVNKFLDM LIKKLKSFDYHQFVDETNDKIREVTQRLNGEIQALE
LPQKAEALKLFLEETKATVAVY LESLQDTKITLIINWLQEALSSASLAH
MKAKFRETLEDTRDRMYQMDIQQELQRYLSL VGQVYSTLVTYISDWWTL
AAKNLTDFAEQYSIQDWAKRMKALVEQGFTVPEIKTILGT MPAFEVSLQ
ALQKATFQTPDFIVPLTDLRIPSVQINFKDLKNIKIPSRFSTPEFTILN
TFHIPSFTIDFVEMKVKIIRTIDQMLNSELQWPVPDIYLRDLKVEDIPLA
RITLPDFR LPEIAIPEFIIPTLNLNDFQVPDLHIPEFQLPHISHTIEVP
TFGKLYSILKIQSPLFT LDANADIGNGTTSANEAGIAASITAKGESKLE
VLNFDFQANAQLSNPKINPLALKESV KFSSKYLRTEHGSEMLFFGNAIE
GKSNTVASLHTEKNTLELSNGVIVKINNQLTLDSN TKYFHKLNIPKLDF
SSQADLRNEIKTLLKAGHIAWTSSGKGSWKWACPRFSDEGTHES QISFT
IEGPLTSFGLSNKINSKHLRVNQNLVYESGSLNFSKLEIQSQVDSQHVGH
SVL TAKGMALFGEGKAEFTGRHDAHLNGKVIGTLKNSLFFSAQPFEITA
STNNEGNLKVRF PLRLTGKIDFLNNYALFLSPSAQQASWQVSARFNQYK
YNQNFSAGNNENIMEAHVGIN GEANLDFLNIPLTIPEMRLPYTIITTPP
LKDFSLWEKTGLKEFLKTTKQSFDLSVKAQ YKKNKHRHSITNPLAVLCE
FISQSIKSFDRHFEKNRNNALDFVTKSYNETKIKFDKYK AEKSHDELPR
TFQIPGYTVPVVNVEVSPFTIEMSAFGYVFPKAVSMPSFSILGSDVRV P
SYTLILPSLELPVLHVPRNLKLSLPDFKELCTISHIFIPAMGNITYDFSF
KSSVITL NTNAELFNQSDIVAHLLSSSSSVIDALQYKLEGTTRLTRKRG
LKLATALSLSNKFVEG SHNSTVSLTTKNMEVSVATTTKAQIPILRMNFK
QELNGNTKSKPTVSSSMEFKYDFNS SMLYSTAKGAVDHKLSLESLTSYF
SIESSTKGDVKGSVLSREYSGTIASEANTYLNSK STRSSVKLQGTSKID
DIWNLEVKENFAGEATLQRIYSLWEHSTKNHLQLEGLFFTNGE HTSKAT
LELSPWQMSALVQVHASQPSSFHDFPDLGQEVALNANTKNQKIRWKNEVR
IH SGSFQSQVELSNDQEKAHLDIAGSLEGHLRFLKNIILPVYDKSLWDF
LKLDVTTSIGR RQHLRVSTAFVYTKNPNGYSFSIPVKVLADKFIIPGLK
LNDLNSVLVMPTFHVPFTDL QVPSCKLDFREIQIYKKLRTSSFALNLPT
LPEVKFPEVDVLTKYSQPEDSLIPFFEIT VPESQLTVSQFTLPKSVSDG
IAALDLNAVANKIADFELPTIIVPEQTIEIPSIKFSVP AGIVIPSFQAL
TARFEVDSPVYNATWSASLKNKADYVETVLDSTCSSTVQFLEYELNV LG
THKIEDGTLASKTKGTFAHRDFSAEYEEDGKYEGLQEWEGKAHLNIKSPA
FTDLHL RYQKDKKGISTSAASPAVGTVGMDMDEDDDFSKWNFYYSPQSS
PDKKLTIFKTELRVR ESDEETQIKVNWEEEAASGLLTSLKDNVPKATGV
LYDYVNKYHWEHTGLTLREVSSKL RRNLQNNAEWVYQGAIRQIDDIDVR
FQKAASGTTGTYQEWKDKAQNLYQELLTQEGQA SFQGLKDNVFDGLVRV
TQEFHMKVKHLIDSLIDFLNFPRFQFPGKPGIYTREELCTMF IREVGTV
LSQVYSKVHNGSEILFSYFQDLVITLPFELRKHKLIDVISMYRELLKDLS
K EAQEVFKAIQSLKTTEVLRNLQDLLQFIFQLIEDNIKQLKEMKFTYLI
NYIQDEINTI FSDYIPYVFKLLKENLCLNLHKFNEFIQNELQEASQELQ
QIHQYIMALREEYFDPSIV GWTVKYYELEEKIVSLIKNLLVALKDFHSE
YIVSASNFTSQLSSQVEQFLHRNIQEYL SILTDPDGKGKEKIAELSATA
QEIIKSQAIATKKIISDYHQQFRYKLQDFSDQLSDYY EKFIAESKRLID
LSIQNYHTFLIYITELLKKLQSTTVMNPYMKLAPGELTIIL

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

515544

Last Updated:

03/04/2022

Links

Characterization Data

SOPs

No SOPs available.

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