Background
Catalog Number:
CPTC-ALCAM-1
Target Antigen:
Activated Leukocyte Cell Adhesion Molecule Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
02/02/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-ALCAM-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (476.2 KB)
CPTC-ALCAM-1 evaluation by the Human Protein Atlas
Result: Negative
This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Background
Catalog Number:
CPTC-ALCAM-2
Target Antigen:
Activated Leukocyte Cell Adhesion Molecule Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-ALCAM-2
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (287.0 KB)
CPTC-ALCAM-2 evaluation by the Human Protein Atlas
Result: Negative
This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
NCI 60 Protein Array
CPTC-ALCAM-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-ALCAM-2 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-ALCAM-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 65 kDa, 63 kDa, 15 kDa, and 34 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-ALCAM-2 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-ALCAM-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 65.1 kDa, 63.7 kDa, 15.1 kDa, and 33.9 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00430
Antigen Name:
Activated Leukocyte Cell Adhesion Molecule Peptide 1
CPTC Name:
CPTC-ALCAM Peptide 1
Aliases:
Activated Leukocyte Cell Adhesion Molecule; MEMD; CD166 Antigen; CD166; Activated Leucocyte Cell Adhesion Molecule; ALCAM
Function:
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. ALCAM (Activated Leukocyte Cell Adhesion Molecule) is a Protein Coding gene. Diseases associated with ALCAM include Stork Bite and Melanoma. Among its related pathways are Mesenchymal Stem Cells and Lineage-specific Markers and L1CAM interactions. Gene Ontology (GO) annotations related to this gene include signaling receptor binding. An important paralog of this gene is MCAM.Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:7760007, PubMed:15496415, PubMed:15048703, PubMed:16352806, PubMed:23169771, PubMed:24945728). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity). CD166_HUMAN,Q13740[Isoform 3]: Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.
Chromosomal Localization:
3q13.11
Accession Number:
NP_001618.2
UniProt Accession Number:
Q13740
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
ALFLETEQLK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1100
Last Updated:
09/24/2020
Links
- EnsEMBL - EnsEMBL ALCAM
- Entrez - Entrez ALCAM
- Uniprot - Uniprot Q13740
Characterization Data
SOPs
No SOPs available.
Don't have Adobe Reader™?
Get it for free at Adobe.com