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AKT Serine/Threonine Kinase 3 Peptide 4

Antibody: CPTC-AKT3-1
Background

Catalog Number:

CPTC-AKT3-1

RRID:

AB_2722019

Target Antigen:

AKT Serine/Threonine Kinase 3 Peptide 4

Isotype:

IgG3

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (511.4 KB)

CPTC-AKT3-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-AKT3-1 antibody with CPTC-AKT3 peptide 4 (NCI ID#00158) as target Click image to enlarge

CPTC-AKT3-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-AKT3-1 antibody with CPTC-AKT3 peptide 4 (NCI ID#00158) as target


Characterization SOP Files

  Immunofluorescence

CPTC-AKT3-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC- AKT3-1 antibody. AKT3 protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (511.4 KB)

CPTC-AKT3-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-AKT3-1 as primary Ab against recombinant AKT3 (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-AKT3-1 Western Blot

Result: Positive

Western Blot using CPTC-AKT3-1 as primary Ab against recombinant AKT3 (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-AKT3-1 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-AKT3-1 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-AKT3-1 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antibody: CPTC-AKT3-2
Background

Catalog Number:

CPTC-AKT3-2

RRID:

AB_2820255

Target Antigen:

AKT Serine/Threonine Kinase 3 Peptide 4

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (492.7 KB)

CPTC-AKT3-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image IHC of CPTC-AKT3-2 applied to lung cancer. Click image to enlarge

CPTC-AKT3-2 Tissue IHC

Result: Positive

IHC of CPTC-AKT3-2 applied to lung cancer.


Characterization SOP Files

  Immunofluorescence

CPTC-AKT3-2 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC- AKT3-2 antibody. AKT3 protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (492.7 KB)

CPTC-AKT3-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-AKT3-2 as primary Ab against recombinant AKT3 (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-AKT3-2 Western Blot

Result: Positive

Western Blot using CPTC-AKT3-2 as primary Ab against recombinant AKT3 (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-AKT-2 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-AKT3-2 Western Blot

Result: Positive

Western Blot using CPTC-AKT-2 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antibody: CPTC-AKT3-3
Background

Catalog Number:

CPTC-AKT3-3

RRID:

AB_2820256

Target Antigen:

AKT Serine/Threonine Kinase 3 Peptide 4

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Phosphorylation

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (304.4 KB)

CPTC-AKT3-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screening results for clone CPTC-AKT3-3. The clone is able to pull down not only the target peptide (panel 1, AKT Serine/Threonine Kinase 3 Peptide 4, RPHFPQF(pS)YSASGRE), but also the following peptides: AKT3 tryptic phosphorylated peptide (RPHFPQF(pS)YSASGR, panel 2) AKT2 Peptide 3 (THFPQF(pS)YSASIRE, panel 3) AKT2 tryptic phosphorylated peptide (THFPQF(pS)YSASIR, panel 4) AKT1 Peptide 3 (RPHFPQF(pS)YSASGTA, panel 5) Click image to enlarge

CPTC-AKT3-3 iMRM (synthetic peptide)

Result: Positive

iMRM screening results for clone CPTC-AKT3-3. The clone is able to pull down not only the target peptide (panel 1, AKT Serine/Threonine Kinase 3 Peptide 4, RPHFPQF(pS)YSASGRE), but also the following peptides:
AKT3 tryptic phosphorylated peptide (RPHFPQF(pS)YSASGR, panel 2)
AKT2 Peptide 3 (THFPQF(pS)YSASIRE, panel 3)
AKT2 tryptic phosphorylated peptide (THFPQF(pS)YSASIR, panel 4)
AKT1 Peptide 3 (RPHFPQF(pS)YSASGTA, panel 5)


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (304.4 KB)

CPTC-AKT3-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-AKT3-3 as primary Ab against recombinant AKT3 (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-AKT3-3 Western Blot

Result: Positive

Western Blot using CPTC-AKT3-3 as primary Ab against recombinant AKT3 (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antibody: CPTC-AKT3-4
Background

Catalog Number:

CPTC-AKT3-4

RRID:

AB_2820257

Target Antigen:

AKT Serine/Threonine Kinase 3 Peptide 4

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

01/04/2023

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (327.0 KB)

CPTC-AKT3-4 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image IHC of CPTC-AKT3-4 antibody applied to colon cancer. Click image to enlarge

CPTC-AKT3-4 Tissue IHC

Result: Positive

IHC of CPTC-AKT3-4 antibody applied to colon cancer.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screening results for clone CPTC-AKT3-4. The clone is able to pull down not only the target peptide (panel 1, AKT Serine/Threonine Kinase 3 Peptide 4, RPHFPQF(pS)YSASGRE), but also the following peptides: AKT3 tryptic phosphorylated peptide (RPHFPQF(pS)YSASGR, panel 2) AKT3 semi-tryptic non-phosphorylated peptide(RPHFPQFSYSASGRE, panel 3) AKT3 tryptic non-phosphorylated peptide(RPHFPQFSYSASGRE, panel 4) AKT2 Peptide 3 (THFPQF(pS)YSASIRE, panel 5) AKT2 tryptic phosphorylated peptide (THFPQF(pS)YSASIR, panel 6) AKT2 semi-tryptic non-phosphorylated peptide (THFPQFSYSASIRE, panel 7) AKT2 tryptic non-phosphorylated peptide (THFPQFSYSASIR, panel 8) AKT1 Peptide 3 (RPHFPQF(pS)YSASGTA, panel 9) AKT1 non-phosphorylated peptide (RPHFPQFSYSASGTA, panel 10) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-AKT3-4 iMRM (synthetic peptide)

Result: Positive

iMRM screening results for clone CPTC-AKT3-4. The clone is able to pull down not only the target peptide (panel 1, AKT Serine/Threonine Kinase 3 Peptide 4, RPHFPQF(pS)YSASGRE), but also the following peptides:
AKT3 tryptic phosphorylated peptide (RPHFPQF(pS)YSASGR, panel 2)
AKT3 semi-tryptic non-phosphorylated peptide(RPHFPQFSYSASGRE, panel 3)
AKT3 tryptic non-phosphorylated peptide(RPHFPQFSYSASGRE, panel 4)
AKT2 Peptide 3 (THFPQF(pS)YSASIRE, panel 5)
AKT2 tryptic phosphorylated peptide (THFPQF(pS)YSASIR, panel 6)
AKT2 semi-tryptic non-phosphorylated peptide (THFPQFSYSASIRE, panel 7)
AKT2 tryptic non-phosphorylated peptide (THFPQFSYSASIR, panel 8)
AKT1 Peptide 3 (RPHFPQF(pS)YSASGTA, panel 9)
AKT1 non-phosphorylated peptide (RPHFPQFSYSASGTA, panel 10)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  Immunofluorescence

CPTC-AKT3-4 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC- AKT3-4 antibody. AKT3 protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (327.0 KB)

CPTC-AKT3-4 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500 Click image to enlarge

CPTC-AKT3-4 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-AKT3-4 as primary Ab against recombinant AKT3 (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-AKT3-4 Western Blot

Result: Positive

Western Blot using CPTC-AKT3-4 as primary Ab against recombinant AKT3 (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-AKT3-4 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-AKT3-4 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-AKT3-4 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: AKT Serine/Threonine Kinase 3 Peptide 4
Background

NCI Identification Number:

00158

Antigen Name:

AKT Serine/Threonine Kinase 3 Peptide 4

CPTC Name:

CPTC-AKT3 Peptide 4

Aliases:

V-Akt Murine Thymoma Viral Oncogene Homolog 3; RAC-PK-Gamma; EC 2.7.11.1; PKB Gamma; STK-2; PKBG; V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma); RAC-Gamma Serine/Threonine Protein Kinase; Protein Kinase B Gamma; Protein Kinase Akt-3; Protein Kinase B; PKB-GAMMA; RAC-Gamma; EC 2.7.11; MPPH2; PRKBG; MPPH

Function:

The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.

AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3) is a Protein Coding gene. Diseases associated with AKT3 include megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 2 and hemimegalencephaly. Among its related pathways are Platelet activation, signaling and aggregation and Gene Expression. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is AKT1.

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.

Akt (Protein kinase B, PKB) is a serine/threonine kinase that plays a key in regulating cell survival, insulin signaling, angiogenesis and tumor formation. Akt is a downstream mediator of the PI 3-K pathway, which results in the recruitment of Akt to the plasma membrane.

Chromosomal Localization:

1q44

Accession Number:

NP_001193658.1

UniProt Accession Number:

Q9Y243

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

RPHFPQF(pS)YSASGRE

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1650

Last Updated:

08/06/2019

Links

Characterization Data

SOPs

No SOPs available.

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