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Aldo-Keto Reductase Family 1 Member C2

Antibody: CPTC-AKR1C2-1
Background

Catalog Number:

CPTC-AKR1C2-1

RRID:

AB_2617220

Target Antigen:

Aldo-Keto Reductase Family 1 Member C2

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Cross Reactivity Data
Click to enlarge image This table shows cross reactivity within the AKR1 family of antigens. Click image to enlarge

Cross Reactivity Data

Result: Positive

This table shows cross reactivity within the AKR1 family of antigens.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (106.4 KB)

CPTC-AKR1C2-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-AKR1C2-1 IHC NCI60

Result: Positive

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-AKR1C2-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-AKR1C2-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-AKR1C2-1 NCI 60 Protein Array

Result: Positive

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500. Click image to enlarge

CPTC-AKR1C2-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-AKR1C2-1 as primary Ab against Ag 10198 (lane 2). Also included are molecular wt. standards (lane 1) and the AKR1C2-1 mAb as control (lane 3). Click image to enlarge

CPTC-AKR1C2-1 Western Blot

Result: Positive

Western Blot using CPTC-AKR1C2-1 as primary Ab against Ag 10198 (lane 2). Also included are molecular wt. standards (lane 1) and the AKR1C2-1 mAb as control (lane 3).


Characterization SOP Files

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Antibody: CPTC-AKR1C2-2
Background

Catalog Number:

CPTC-AKR1C2-2

RRID:

AB_2617221

Target Antigen:

Aldo-Keto Reductase Family 1 Member C2

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

10/24/2019

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Cross Reactivity Data
Click to enlarge image This table shows cross reactivity within the AKR1 family of antigens. Click image to enlarge

Cross Reactivity Data

Result: Positive

This table shows cross reactivity within the AKR1 family of antigens.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (93.0 KB)

CPTC-AKR1C2-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-AKR1C2-2 IHC NCI60

Result: Positive

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-AKR1C2-2 IHC Tissue

Result: Positive

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-AKR1C2-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-AKR1C2-2 NCI 60 Protein Array

Result: Positive

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-AKR1C2-2 as primary Ab against Ag 10198 (lane 2). Also included are molecular wt. standards (lane 1) and the AKR1C2-2 mAb as control (lane 3). Click image to enlarge

CPTC-AKR1C2-2 Western Blot

Result: Positive

Western Blot using CPTC-AKR1C2-2 as primary Ab against Ag 10198 (lane 2). Also included are molecular wt. standards (lane 1) and the AKR1C2-2 mAb as control (lane 3).


Characterization SOP Files

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Antibody: CPTC-AKR1C2-3
Background

Catalog Number:

CPTC-AKR1C2-3

RRID:

AB_2617222

Target Antigen:

Aldo-Keto Reductase Family 1 Member C2

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

10/24/2019

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Cross Reactivity Data
Click to enlarge image This table shows cross reactivity within the AKR1 family of antigens. Click image to enlarge

Cross Reactivity Data

Result: Positive

This table shows cross reactivity within the AKR1 family of antigens.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (91.3 KB)

CPTC-AKR1C2-3 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-AKR1C2-3 IHC NCI60

Result: Positive

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-AKR1C2-3 IHC Tissue

Result: Positive

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-AKR1C2-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-AKR1C2-3 NCI 60 Protein Array

Result: Positive

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-AKR1C2-3 as primary Ab against Ag 10198 (lane 2). Also included are molecular wt. standards (lane 1) and the AKR1C2-3 mAb as control (lane 3). Click image to enlarge

CPTC-AKR1C2-3 Western Blot

Result: Positive

Western Blot using CPTC-AKR1C2-3 as primary Ab against Ag 10198 (lane 2). Also included are molecular wt. standards (lane 1) and the AKR1C2-3 mAb as control (lane 3).


Characterization SOP Files

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Antigen: Aldo-Keto Reductase Family 1 Member C2
Background

NCI Identification Number:

10198

Antigen Name:

Aldo-Keto Reductase Family 1 Member C2

CPTC Name:

CPTC-AKR1C2

Aliases:

AKR1C2; 3-alpha-HSD3; AKR1C-pseudo; BABP; DD; DD-2; DD/BABP; DD2; DDH2; EC 1.-.-.-,EC 1.3.1.20,EC 1.1.1.213; HAKRD; HBAB; MCDR2; OTTHUMP00000018995; OTTHUMP00000044759

Function:

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability.

Chromosomal Localization:

10p15-p14

Expression System:

E. Coli

Accession Number:

BC007024

UniProt Accession Number:

P52895

DNA Source:

HIP : HsCD00004147

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

37548

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEAVKLAIEAGFH
HIDSAHVYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSNSHRPELVRP
ALERSLKNLQLDYVDLYLIHFPVSVKPGEEVIPKDENGKILFDTVDLCAT
WEAMEKCKDAGLAKSIGVSNFNHRLLEMILNKPGLKYKPVCNQVECHPYF
NQRKLLDFCKSKDIVLVAYSALGSHREEPWVDPNSPVLLEDPVLCALAKK
HKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTSEEMKAID
GLNRNVRYLTLDIFAGPPNYPFSDEY

Native Sequence:

MDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEAVKLAIEAGFHHID
SAHVYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSNSHRPELVRPALE
RSLKNLQLDYVDLYLIHFPVSVKPGEEVIPKDENGKILFDTVDLCATWEA
MEKCKDAGLAKSIGVSNFNHRLLEMILNKPGLKYKPVCNQVECHPYFNQR
KLLDFCKSKDIVLVAYSALGSHREEPWVDPNSPVLLEDPVLCALAKKHKR
TPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTSEEMKAIDGLN
RNVRYLTLDIFAGPPNYPFSDEY

Calculated Isoelectric Point:

7.12

Molecular Weight:

37008

Last Updated:

08/22/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of CPTC-AKR1C2 (Ag 10198),with molecular weight standards in lane 1
Click image to enlarge

Ag 10198 PAGE

PAGE of CPTC-AKR1C2 (Ag 10198),with molecular weight standards in lane 1

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SOPs

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