Background
Catalog Number:
CPTC-ABCB1-1
Target Antigen:
ATP Binding Cassette Subfamily B Member 1 Peptide 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
03/08/2022
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
IHC Tissue
CPTC-ABCB1-1 IHC Tissue
Result: Positive
Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-ABCB1-1. Titer: 1:5000
Characterization SOP Files
Indirect ELISA
CPTC-ABCB1-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-ABCB1-1 NCI 60 Protein Array
Result: Positive
Protein Array in which CPTC-ABCB1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Background
Catalog Number:
CPTC-ABCB1-2
RRID:
AB_2814777
Target Antigen:
ATP Binding Cassette Subfamily B Member 1 Peptide 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/01/2022
Antigen Recognition(s):
Peptide, Endogenous
Purchase
- DSHB CPTC-ABCB1-2
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (474.5 KB)
CPTC-ABCB1-2 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC Tissue
CPTC-ABCB1-2 IHC Tissue
Result: Positive
Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-ABCB1-2. Titer: 1:100
Characterization SOP Files
Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (474.5 KB)
CPTC-ABCB1-2 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-ABCB1-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-ABCB1-2 NCI 60 Protein Array
Result: Positive
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Background
Catalog Number:
CPTC-ABCB1-3
Target Antigen:
ATP Binding Cassette Subfamily B Member 1 Peptide 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
10/07/2021
Antigen Recognition(s):
Peptide, Endogenous
Characterization Data [Compare Characterization Data]
Indirect ELISA
CPTC-ABCB1-3 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-ABCB1-3 NCI 60 Protein Array
Result: Positive
Protein Array in which CPTC-ABCB1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Background
NCI Identification Number:
00257
Antigen Name:
ATP Binding Cassette Subfamily B Member 1 Peptide 1
CPTC Name:
CPTC-ABCB1 Peptide 1
Aliases:
ATP Binding Cassette Subfamily B Member 1; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1; Multidrug Resistance Protein 1; Colchicin Sensitivity; P-Glycoprotein 1; EC 3.6.3.44; MDR1; PGY1; ATP-Binding Cassette Sub-Family B Member 1; P Glycoprotein; CD243 Antigen; EC 3.6.3; ABC20; CD243; GP170; P-GP; CLCS
Function:
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
ABCB1 (ATP Binding Cassette Subfamily B Member 1) is a Protein Coding gene. Diseases associated with ABCB1 include Colchicine Resistance and Inflammatory Bowel Disease 13. Among its related pathways are Abacavir transport and metabolism and Vinka Alkaloid Pathway, Pharmacokinetics. Gene Ontology (GO) annotations related to this gene include transporter activity and ATPase activity, coupled to transmembrane movement of substances. An important paralog of this gene is ABCB4.
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
ATP-binding cassette (ABC) transporters form one of the largest groups of paralogous protein families that have a wide diversity of functions and substrates. Specificity has been shown for small and large molecules, highly charged molecules and highly hydrophobic systems.
Chromosomal Localization:
7q21.12
Accession Number:
NM_000927.4
UniProt Accession Number:
P08183
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
FGEMTDIFANAGNLEDLMSNI
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
2310
Last Updated:
10/01/2019
Links
- EnsEMBL - EnsEMBL ABCB1
- Entrez - Entrez ABCB1
- Uniprot - Uniprot P08183
Characterization Data
SOPs
No SOPs available.
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