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2-methylsuccinyl-cysteine Peptide 1

Antibody: CPTC-2MeSC-1
Background

Catalog Number:

CPTC-2MeSC-1

Target Antigen:

2-methylsuccinyl-cysteine Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

02/10/2026

Antigen Recognition(s):

Peptide, Synthetic Compound

Publications
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-2MeSC-1 and BSA-conjugated Itaconate peptide were measured using biolayer interferometry. CPTC-2MeSc-1 mouse antibody was captured onto Protein G biosensor. The BSA-conjugated Itaconate peptide at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. All binding data were analyzed globally using a 1:1 fitting. Click image to enlarge

CPTC-2MeSC-1 Affinity and Kinetics (Bio-Layer Interferometry) 

Result: High Binding

Affinity and binding kinetics of CPTC-2MeSC-1 and BSA-conjugated Itaconate peptide were measured using biolayer interferometry. CPTC-2MeSc-1 mouse antibody was captured onto Protein G biosensor. The BSA-conjugated Itaconate peptide at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. All binding data were analyzed globally using a 1:1 fitting.


Characterization SOP Files

  Immunofluorescence

CPTC-2MeSC-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of C57BL/6J wild-type (WT) and on the C57BL/6J genetic background Irg1−/− knock-out (KO) mouse bone marrow derived macrophages (BMDMs) differentiated with 40 ng/mL Macrophage colony-stimulating factor (MCSF) for 6 days and stimulated with 500 ng/mL Lipopolysaccharide (LPS) for 24 hrs. using CPTC-2MeSC-1 as primary antibody. 2MeSC protein expression shows no localization.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-2MeSC-1 as primary antibody against BSA-conjugated Itaconate peptide, coated on the plate and detected using the goat anti- mouse antibody and TMB. Click image to enlarge

CPTC-2MeSC-1 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-2MeSC-1 as primary antibody against BSA-conjugated Itaconate peptide, coated on the plate and detected using the goat anti- mouse antibody and TMB.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Automated western blot using CPTC-2MeSCe-1 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is able to recognize the BSA conjugated Itaconate, without any crossreactivity with BSA. Click image to enlarge

CPTC-2MeSCe-1 Simple Western (BSA Conjugated peptide)

Result: Positive

Automated western blot using CPTC-2MeSCe-1 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is able to recognize the BSA conjugated Itaconate, without any crossreactivity with BSA.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-2MeSCe-1 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is able to recognize the BSA conjugated Itaconate, without any cross-reactivity with BSA. Click image to enlarge

CPTC-2MeSCe-1 Western Blot (BSA Conjugated peptide)

Result: Positive

Western blot using CPTC-2MeSCe-1 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is able to recognize the BSA conjugated Itaconate, without any cross-reactivity with BSA.


Characterization SOP Files

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Antibody: CPTC-2MeSC-2
Background

Catalog Number:

CPTC-2MeSC-2

Target Antigen:

2-methylsuccinyl-cysteine Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

02/10/2026

Antigen Recognition(s):

Peptide, Synthetic Compound

Publications
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-2MeSC-2 and BSA-conjugated Itaconate peptide were measured using biolayer interferometry. CPTC-2MeSc-2 mouse antibody was captured onto Protein G biosensor. The BSA-conjugated Itaconate peptide at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. All binding data were analyzed globally using a 1:1 fitting. Click image to enlarge

CPTC-2MeSC-2 Affinity and Kinetics (Bio-Layer Interferometry) 

Result: High Binding

Affinity and binding kinetics of CPTC-2MeSC-2 and BSA-conjugated Itaconate peptide were measured using biolayer interferometry. CPTC-2MeSc-2 mouse antibody was captured onto Protein G biosensor. The BSA-conjugated Itaconate peptide at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. All binding data were analyzed globally using a 1:1 fitting.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-2MeSC-2 and BSA-conjugated Itaconate peptide were measured using surface plasmon resonance. CPTC-2MeSC-2 antibody was captured onto a Series S Protein G biosensor chip. The BSA-conjugated Itaconate peptide was titrated over the antibody surface at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM , 0.25 nM, and 0.0625 nM. Binding data were double-referenced and analyzed globally to a 1:1 fitting model. Click image to enlarge

CPTC-2MeSC-2 Affinity and Kinetics (Surface Plasmon Resonance) 

Result: High Binding

Affinity and binding kinetics of CPTC-2MeSC-2 and BSA-conjugated Itaconate peptide were measured using surface plasmon resonance. CPTC-2MeSC-2 antibody was captured onto a Series S Protein G biosensor chip. The BSA-conjugated Itaconate peptide was titrated over the antibody surface at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM , 0.25 nM, and 0.0625 nM. Binding data were double-referenced and analyzed globally to a 1:1 fitting model.


Characterization SOP Files

  Immunofluorescence

CPTC-2MeSC-2 Immunofluorescence

Result: Negative

Immunofluorescence staining of C57BL/6J wild-type (WT) and on the C57BL/6J genetic background Irg1−/− knock-out (KO) mouse bone marrow derived macrophages (BMDMs) differentiated with 40 ng/mL Macrophage colony-stimulating factor (MCSF) for 6 days and stimulated with 500 ng/mL Lipopolysaccharide (LPS) for 24 hrs. using CPTC-2MeSC-2 as primary antibody. 2MeSC protein expression shows no localization.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-2MeSC-2 as primary antibody against BSA-conjugated Itaconate peptide, coated on the plate and detected using the goat anti-mouse antibody and TMB. Click image to enlarge

CPTC-2MeSC-2 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-2MeSC-2 as primary antibody against BSA-conjugated Itaconate peptide, coated on the plate and detected using the goat anti-mouse antibody and TMB.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Automated western blot using CPTC-2MeSCe-2 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is able to recognize the BSA conjugated Itaconate, without any crossreactivity with BSA. Click image to enlarge

CPTC-2MeSCe-2 Simple Western (BSAConjugated peptide)

Result: Positive

Automated western blot using CPTC-2MeSCe-2 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is able to recognize the BSA conjugated Itaconate, without any crossreactivity with BSA.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-2MeSCe-2 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is able to recognize the BSA conjugated Itaconate, without any cross-reactivity with BSA. Click image to enlarge

CPTC-2MeSCe-2 Western Blot (BSA Conjugated peptide)

Result: Positive

Western blot using CPTC-2MeSCe-2 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is able to recognize the BSA conjugated Itaconate, without any cross-reactivity with BSA.


Characterization SOP Files

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Antibody: CPTC-2MeSC-3
Background

Catalog Number:

CPTC-2MeSC-3

Target Antigen:

2-methylsuccinyl-cysteine Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

02/10/2026

Antigen Recognition(s):

Synthetic Compound

Publications
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry

CPTC-2MeSC-3 Affinity and Kinetics (Bio-Layer Interferometry) 

Result: No Binding

Affinity and binding kinetics of CPTC-2MeSC-3 and BSA-conjugated Itaconate peptide were measured using biolayer interferometry. CPTC-2MeSc-3 mouse antibody was captured onto Protein G biosensor. The BSA-conjugated Itaconate peptide at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. All binding data were analyzed globally using a 1:1 fitting.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-2MeSC-3 and BSA-conjugated Itaconate peptide was measured using surface plasmon resonance. CPTC-2MeSC-3 antibody was captured onto a Series S Protein G biosensor chip. The BSA-conjugated Itaconate peptide was titrated over the antibody surface at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM , 0.25 nM, and 0.0625 nM. Binding data were double-referenced and analyzed globally to a 1:1 fittingmodel. No binding was observed. Click image to enlarge

CPTC-2MeSC-3 Affinity and Kinetics (Surface Plasmon Resonance) 

Result: No Binding

Affinity and binding kinetics of CPTC-2MeSC-3 and BSA-conjugated Itaconate peptide was measured using surface plasmon resonance. CPTC-2MeSC-3 antibody was captured onto a Series S Protein G biosensor chip. The BSA-conjugated Itaconate peptide was titrated over the antibody surface at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM , 0.25 nM, and 0.0625 nM. Binding data were double-referenced and analyzed globally to a 1:1 fittingmodel. No binding was observed.


Characterization SOP Files

  Immunofluorescence

CPTC-2MeSC-3 Immunofluorescence

Result: Negative

Immunofluorescence staining of C57BL/6J wild-type (WT) and on the C57BL/6J genetic background Irg1−/− knock-out (KO) mouse bone marrow derived macrophages (BMDMs) differentiated with 40 ng/mL Macrophage colony-stimulating factor (MCSF) for 6 days and stimulated with 500 ng/mL Lipopolysaccharide (LPS) for 24 hrs. using CPTC-2MeSC-3 as primary antibody. 2MeSC protein expression shows no localization.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-2MeSC-3 as primary antibody against BSA-conjugated Itaconate peptide, coated on the plate and detected using the goat anti-mouse antibody and TMB. No binding was observed. Click image to enlarge

CPTC-2MeSC-3 Indirect ELISA

Result: No Binding

Indirect ELISA using CPTC-2MeSC-3 as primary antibody against BSA-conjugated Itaconate peptide, coated on the plate and detected using the goat anti-mouse antibody and TMB. No binding was observed.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Automated western blot using CPTC-2MeSCe-3 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is able to recognize very weakly the BSA conjugated Itaconate, and shows no crossreactivity with BSA. Click image to enlarge

CPTC-2MeSCe-3 Simple Western (BSA Conjugated peptide)

Result: Presumed Positive (with additional bands)

Automated western blot using CPTC-2MeSCe-3 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is able to recognize very weakly the BSA conjugated Itaconate, and shows no crossreactivity with BSA.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-2MeSCe-3 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is not able to recognize the BSA conjugated Itaconate, and shows no cross-reactivity with BSA. Click image to enlarge

CPTC-2MeSCe-3 Western Blot (BSA Conjugated peptide)

Result: Negative

Western blot using CPTC-2MeSCe-3 as primary antibody against BSA conjugated Itaconate and BSA (negative control). The antibody is not able to recognize the BSA conjugated Itaconate, and shows no cross-reactivity with BSA.


Characterization SOP Files

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Antigen: 2-methylsuccinyl-cysteine Peptide 1
Background

NCI Identification Number:

00533

Antigen Name:

2-methylsuccinyl-cysteine Peptide 1

CPTC Name:

CPTC-2MeSC Peptide 1

Aliases:

S-(2-methylsuccinic acid)-cysteine; itaconate-cysteine conjugate; S-conjugated itaconate

Function:

2-methyl-succination is a stable post-translational modification of cysteine residues, which changes redox state in the cell, alters protein function or metabolic rate in response to an inflammatory insult, infection or changing intracellular environment. 2-methyl-succination occurs when the Krebs cycle intermediate, itaconate, reacts with cysteine yielding S-(2-methylsuccinic acid)-cysteine.A wide range of proteins are subject to 2-methyl-succination, including enzymes and regulatory proteins and this process has been shown to have roles in tuning inflammatory response, metabolism and other functions.2MeSC therefore may serve as a biomarker of mitochondrial stress or dysfunction in chronic inflammation including infectious disease, obesity, diabetes, and cancer.https://hmdb.ca/metabolit

Chromosomal Localization:

Accession Number:

CAS No.: 498-21-5

UniProt Accession Number:

DNA Source:

N/A

Immunogen:

Synthetic Compound

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

C5H6O4

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

130

Last Updated:

07/27/2022

Links

Characterization Data

SOPs

No SOPs available.

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