CD74 Molecule Peptide 1


Background

Catalog Number:

CPTC-CD74-1

RRID:

AB_2889054

Target Antigen:

CD74 Molecule Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/08/2022

Antigen Recognition(s):

Peptide, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (295.1 KB)

CPTC-CD74-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer  showing cytoplasmic and membranous staining using Antibody CPTC-CD74-1. Titer: 1:50 Click image to enlarge

CTPC-CD74-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-CD74-1. Titer: 1:50


  Immunofluorescence

CPTC-CD74-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-CD74-1 Ab shows no localization of CD74 protein.


  NCI 60 Protein Array

CTPC-CD74-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD74-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates.  Expected molecular weight - 33.3 kDa.  Molecular weight standards are also included (lane 1). Inconclusive data. Click image to enlarge

CPTC-CD74-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-CD74-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 33.3 kDa. Molecular weight standards are also included (lane 1). Inconclusive data.


  Western Blot - Cell Lysate
Click to enlarge image Western blot using CPTC-CD74-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates.  Expected molecular weight 34 kDa.  Molecular weight standards (MW Stds.) are also included (lane 1).  Positive for Jurkat and MCF7.  Negative/ inconclusive data for the other cell lines. Click image to enlarge

CPTC-CD74-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-CD74-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 34 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for Jurkat and MCF7. Negative/ inconclusive data for the other cell lines.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-CD74-1 as primary antibody against human recombinant protein CD74 , major histocompatibility complex, class II invariant chain (lane 2). Expected molecular weight - 33.3 kDa.  Molecular weight standards are also included (lane 1). Inconclusive data. Click image to enlarge

CPTC-CD74-1 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-CD74-1 as primary antibody against human recombinant protein CD74 , major histocompatibility complex, class II invariant chain (lane 2). Expected molecular weight - 33.3 kDa. Molecular weight standards are also included (lane 1). Inconclusive data.


Characterization SOP Files

Background

NCI Identification Number:

00358

Antigen Name:

CD74 Molecule Peptide 1

CPTC Name:

CPTC-CD74 Peptide 1

Aliases:

CD74 Molecule; CD74 Antigen (Invariant Polypeptide Of Major Histocompatibility Complex, Class II Antigen-Associated); CD74 Molecule, Major Histocompatibility Complex, Class II Invariant Chain; HLA Class II Histocompatibility Antigen Gamma Chain; HLA-DR Antigens-Associated Invariant Chain; Gamma Chain Of Class II Antigens; Ia-Associated Invariant Chain; MHC HLA-DR Gamma Chain; HLA-DR-Gamma; DHLAG; P33; Ia Antigen-Associated Invariant Chain; CD74 Antigen; Ia-GAMMA; HLADG; CD74; II; Ii

Function:

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. CD74 (CD74 Molecule) is a Protein Coding gene. Diseases associated with CD74 include Mucinous Lung Adenocarcinoma and Lung Cancer Susceptibility 3. Among its related pathways are Tuberculosis and Cell surface interactions at the vascular wall. Gene Ontology (GO) annotations related to this gene include identical protein binding and amyloid-beta binding.Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. Class-II-associated invariant chain peptide]: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.

Chromosomal Localization:

5q33.1

Accession Number:

NP_001020330.1

UniProt Accession Number:

P04233

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CQEEVSHIPAVHPGSFRPK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2090

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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